<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vguit</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Воронежского государственного университета инженерных технологий</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of the Voronezh State University of Engineering Technologies</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2226-910X</issn><issn pub-type="epub">2310-1202</issn><publisher><publisher-name>VSUET</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.20914/2310-1202-2017-3-174-179</article-id><article-id custom-type="elpub" pub-id-type="custom">vguit-1579</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Биотехнология и бионанотехнология</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Biotechnology, bionanotechnology and sugary products technology</subject></subj-group></article-categories><title-group><article-title>Влияние бактериальных культур на сохранность зеленых кормов</article-title><trans-title-group xml:lang="en"><trans-title>The influence of bacterial cultures on the conservation of green fodders</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мальцева</surname><given-names>О. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Maltseva</surname><given-names>O. Yu.</given-names></name></name-alternatives><email xlink:type="simple">ksenia2002@list.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мещерякова</surname><given-names>О. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Meshcheryakova</surname><given-names>O. L.</given-names></name></name-alternatives><email xlink:type="simple">gawshina@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Новикова</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Novikova</surname><given-names>I. V.</given-names></name></name-alternatives><email xlink:type="simple">noviv@list.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Степанова</surname><given-names>Д. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Stepanova</surname><given-names>D. S.</given-names></name></name-alternatives><email xlink:type="simple">dariastepanova-95@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Степанова</surname><given-names>М. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Stepanova</surname><given-names>M. S.</given-names></name></name-alternatives><email xlink:type="simple">mariastepanova95@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Корнеева</surname><given-names>О. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Korneeva</surname><given-names>O. S.</given-names></name></name-alternatives><email xlink:type="simple">korneeva-olgas@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Воронежский государственный университет инженерных технологий</institution></aff><aff xml:lang="en"><institution>Voronezh state university of engineering technologies</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2017</year></pub-date><pub-date pub-type="epub"><day>14</day><month>11</month><year>2017</year></pub-date><volume>79</volume><issue>3</issue><fpage>174</fpage><lpage>179</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Мальцева О.Ю., Мещерякова О.Л., Новикова И.В., Степанова Д.С., Степанова М.С., Корнеева О.С., 2017</copyright-statement><copyright-year>2017</copyright-year><copyright-holder xml:lang="ru">Мальцева О.Ю., Мещерякова О.Л., Новикова И.В., Степанова Д.С., Степанова М.С., Корнеева О.С.</copyright-holder><copyright-holder xml:lang="en">Maltseva O.Y., Meshcheryakova O.L., Novikova I.V., Stepanova D.S., Stepanova M.S., Korneeva O.S.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vestnik-vsuet.ru/vguit/article/view/1579">https://www.vestnik-vsuet.ru/vguit/article/view/1579</self-uri><abstract><p>Для получения силосной закваски растительного сырья на основе бактериальных монокультур были тщательно изучены свойства и выбраны штаммы молочнокислых и пропионовокислых бактерий, наиболее полно удовлетворяющие требованиям, предъявляемым к консервирующим препаратам. Были выбраны следующие штаммы: Bifidobacterium bifidum N1 в виде лиофилизата, Propionibacterium freudenreichiisub sp. shermanii – КМ 186 в виде жидкого препарата, Lactobacillus plantarum 8P-A3 в виде лиофилизата. Для получения данного бактериального концентрата была проведена серия опытов по подбору необходимых концентраций для каждого вида силосуемого сырья. Положительным результатом считали отсутствие посторонней микрофлоры в заложенной на хранение биомассе по истечении двух месяцев. О качестве заготавливаемого силоса судили по содержанию органических кислот в травянистой массе. Основным показателем эффективности применения молочнокислых бактерий для закваски является накопление молочной кислоты, пропионовокислых – пропионовой кислоты. Содержание кислот при силосовании трудносилосуемых (амарант красный в фазе молочно-восковой спелости, лебеда) и легкосилосуемых культур (кукуруза в начале фазы восковой спелости (конец фазы молочно-восковой спелости), подсолнечник однолетний масличный в начале цветения) определяли в соответствии с ГОСТ. Высокое содержание молочной и пропионовой кислот гарантирует хорошую сохранность силоса. Их низкое содержание означает недостаток сахаров в массе или же указывает на то, что в массу проникает кислород.</p></abstract><trans-abstract xml:lang="en"><p>Properties of microbial cultures were carefully studied and strains of lactic and propionate bacteria, which meet requirements imposed to the preserving medicines, are chosen. The following strains were selected: Bifidobacterium bifidum N1 as a lyophilizate, Propionibacterium freudenreichii subsp. shermanii - KM 186 in the form of a liquid preparation, Lactobacillus plantarum 8P-A3 in the form of lyophilizate. To obtain this bacterial concentrate, a series of experiments was conducted to select the necessary concentrations and to create the optimal composition of the starter for each type of silicate raw material. A positive result was the absence of extraneous micro flora in the stored biomass after a month. The quality of harvested silage was judged by the content of organic acids in the herbaceous mass. The main indicator of the effectiveness of lactic acid bacteria for leaven is the accumulation of lactic acid, propionic acid - propionic acid. The content of acids in the siloing of the labor-prone (amaranth red in the phase of the milk-wax ripeness, quinoa) and light-sown crops (corn at the beginning of the phase of wax ripeness (end of the phase of milky wax ripeness), sunflower one-year-old oilseed at the beginning of flowering) was determined in accordance with GOST. The high content of lactic and propionic acids guarantees good preservation of the silage. Their low content means a lack of sugars in the mass or indicates that oxygen penetrates into the mass.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>силосование</kwd><kwd>закваска</kwd><kwd>травянистые корма</kwd><kwd>сельское хозяйство</kwd><kwd>молочнокислые бактерии</kwd><kwd>пропионовокислые бактерии</kwd></kwd-group><kwd-group xml:lang="en"><kwd>silage</kwd><kwd>leaven</kwd><kwd>herbaceous fodder</kwd><kwd>agriculture</kwd><kwd>lactic acid bacteria</kwd><kwd>propionic acid bacteria</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Иванов Д.В. Современные технологии и технические средства приготовления силосованных кормов: учебное пособие. Ставрополь: АГРУС, 2014.</mixed-citation><mixed-citation xml:lang="en">1	Ivanov D.V. Sovremennye tekhnologii I tekhnicheskie sredstva [Modern technologies and technical means of preparation of silage forages] Stavropol, AGRUS, 2014. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Семенихина В.Ф., Будрик В.Г., Рожкова И.В. и др. Разработка технологий заквасок прямого внесения // Сборник научных трудов. «Научное обеспечение молочной промышленности». 2010.</mixed-citation><mixed-citation xml:lang="en">2	Semenikhin V.F., Budrik V.G., Rozhkova I.V. et al. Development of technologies for starter cultures of direct application. Nauchnoe obespechenie molochnoi promyshlennosti [Collection of scientific works. "Scientific provision of the dairy industry"] Moscow, VNIMI, 2010. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Абраскова С.В., Шашко Ю.К., Шашко М.Н. Биологическая безопасность кормов. Минск: Беларус. наука, 2013. 257 с.</mixed-citation><mixed-citation xml:lang="en">3	Abraskova S.V., Shashko Yu. K., Shashko M.N. Biologicheskaya bezopasnost’ kormov [Biological safety of fodder] Minsk, Belarus Science, 2013. 257 p. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Чичерин И.Ю., Погорельский И.П., Лундовских И.А., Малов А.А. и др. Динамика содержания лактобацилл, микробных метаболитов и антибактериальной активности растущей культуры LactobacillusPlantarum 8P-A3 // Журнал инфектологии. 2013.</mixed-citation><mixed-citation xml:lang="en">4	Chicherin I.Yu., Pogorelsky I.P., Lundovskikh I.A., Malov A.A. et al. Dynamics of the content of lactobacilli, microbial metabolites and antibacterial activity of the growing culture of Lactobacillus Plantarum 8P-A3. Zhurnal infektologii [Journal of Infectology] 2013. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Denkova R., Denkova Z., Yanakieva V., Blazheva D. Antimicrobial activity of propionic lactobacilli, bifidobacteria and propionic acid bacteria, isolated from different sources //Formatex. 2013. V.2. P. 857–864.</mixed-citation><mixed-citation xml:lang="en">5	Denkova R., Denkova Z., Yanakieva V., Blazheva D. Antimicrobial activity of propionic lactobacilli, bifidobacteria and propionic acid bacteria, isolated from different sources. Formatex. 2013.  vol. 2. pp. 857–864.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Bevilacqua A., Cagnazzo M.T., Caldarola C., Ciuffreda E. et al. Bifidobacteria as Potential Functional Starter Cultures // SciRes. 2012. V.3. P.55–63.</mixed-citation><mixed-citation xml:lang="en">6	Bevilacqua A., Cagnazzo M.T., Caldarola C., Ciuffreda E. et al. Bifidobacteria as Potential Functional Starter Cultures. SciRes. 2012. vol. 3. pp.55–63.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">ГОСТ 23637–90. Сенаж. Технические условия. Определение массовой доли органических кислот методом Леппера-Флига.</mixed-citation><mixed-citation xml:lang="en">7	GOST 23637–90. Senazh. Tekhnicheskie usloviya [State standard 23637–90. Haylage. Technical conditions. Determination of the mass fraction of organic acids by the Le]per-Flig method] Moscow, Standartinform Publ., 1991. 10 p. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">ГОСТ 10444.11–2013 (ISO 15214:1998). Микробиология пищевых продуктов и кормов для животных.</mixed-citation><mixed-citation xml:lang="en">8	GOST 10444.11–2013 (ISO 15214: 1998). Mikrobiologiya pishchevykh produktov [State standard 10444.11–2013. Microbiology of food and animal feeding stuffs] (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Bhavya V. et al. Effect of clusterbean genotypes (Cyamopsistetragonoloba L.) with different seed rate on green fodder yield //International Journal of Tropical Agriculture. 2016. V. 34. №. 4. P. 961-964.</mixed-citation><mixed-citation xml:lang="en">9	Bhavya V. et al. Effect of clusterbean genotypes (Cyamopsistetragonoloba L.) with different seed rate on green fodder yield. International Journal of Tropical Agriculture. 2016. vol. 34. no. 4. pp. 961-964.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Mewes M. et al. A systematic approach for assessing spatially and temporally differentiated opportunity costs of biodiversity conservation measures in grasslands //Agricultural Systems. 2015. V. 137. P. 76-88.</mixed-citation><mixed-citation xml:lang="en">10	Mewes M. et al. A systematic approach for assessing spatially and temporally differentiated opportunity costs of biodiversity conservation measures in grasslands. Agricultural Systems.  2015. vol. 137. pp. 76-88.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Mupangwa W., Thierfelder C. Intensification of conservation agriculture systems for increased livestock feed and maize production in Zimbabwe // International Journal of Agricultural Sustainability. 2014. V. 12. №. 4. P. 425-439.</mixed-citation><mixed-citation xml:lang="en">11	Mupangwa W., Thierfelder C. Intensification of conservation agriculture systems for increased livestock feed and maize production in Zimbabwe. International Journal of Agricultural Sustainability.  2014. vol. 12.  no.. 4. pp. 425-439.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
