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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vguit</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Воронежского государственного университета инженерных технологий</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of the Voronezh State University of Engineering Technologies</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2226-910X</issn><issn pub-type="epub">2310-1202</issn><publisher><publisher-name>VSUET</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.20914/2310-1202-2018-3-205-211</article-id><article-id custom-type="elpub" pub-id-type="custom">vguit-1951</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Пищевая  биотехнология</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Food biotechnology</subject></subj-group></article-categories><title-group><article-title>Культивирование Leuconostoc mezenteroides на гидролизате биомассы кукурузы</article-title><trans-title-group xml:lang="en"><trans-title>Growth studies of Leuconostoc mesenteroides on corn biomass based substrates</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хусаинов</surname><given-names>И. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Khusainov</surname><given-names>I. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>ассистент, кафедра пищевой инженерии малых предприятий, ул. К. Маркса, 68, г. Казань, 420015, Республика Татарстан, Россия</p></bio><bio xml:lang="en"><p>assistant, food engineering in small enterprises department, K. Marks st. 68, 420015, Russia</p></bio><email xlink:type="simple">Fortes16@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Канарский</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kanarskiy</surname><given-names>A. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>д.т.н., профессор, кафедра пищевой биотехнологии, ул. К. Маркса, 68, г. Казань, 420015, Республика Татарстан, Россия</p></bio><bio xml:lang="en"><p>Dr. Sci. (Engin.), professor, department of food biotechology, K. Marks st. 68, 420015, Russia</p></bio><email xlink:type="simple">alb@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Казанский национальный исследовательский технологический университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Kazan national research technological university</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>26</day><month>09</month><year>2018</year></pub-date><volume>80</volume><issue>3</issue><fpage>205</fpage><lpage>211</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Хусаинов И.А., Канарский А.В., 2018</copyright-statement><copyright-year>2018</copyright-year><copyright-holder xml:lang="ru">Хусаинов И.А., Канарский А.В.</copyright-holder><copyright-holder xml:lang="en">Khusainov I.A., Kanarskiy A.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vestnik-vsuet.ru/vguit/article/view/1951">https://www.vestnik-vsuet.ru/vguit/article/view/1951</self-uri><abstract><p>Бактерии Leuconostoc mesenteroides – известный промышленный продуцент внеклеточного бактериального полисахарида декстрана. В качестве основного сырья для промышленного культивирования этих микроорганизмов применяется меласса, сахароза которой является источником углерода и энергии для бактерий, а также предшественником биосинтеза декстрана. Известно, что бактерии рода Leuconostoc являются гетероферментативными микроорганизмами и способны утилизировать различные углеводы. Целью данного исследования являлось изучение влияния состава среды на основе гидролизата кукурузы и условий культивирования на выход биомассы бактерий и концентрацию внеклеточного полисахарида в культуральной жидкости. Гидролизат представляет собой продукт хемо-ферментативной обработки растительной лигноцеллюлозной биомассы кукурузы и содержит такие моносахара как глюкоза, арабиноза и ксилоза.Параметрами состава среды являлись концентрация углеводов гидролизата в виде редуцирующих веществ (РВ), концентрация азота (белкового и небелкового), сахарозы и наличие минеральных веществ. Параметры процесса культивирования – рН и температура. Согласно экспериментальным данным, максимальные выходы биомассы бактерий от РВ "*"(9,3 и 8,5%) достигались на комбинированных средах с содержанием сахарозы 0,2% и соли натрия (фосфата натрия), соответственно. Наибольшиеудельные скорости роста микроорганизмов (0,0840 и 0,0746) получены на комбинированной среде с содержанием 0,15 и 0,3% неорганического азота соответственно. Наибольшие концентрации внеклеточных полисахаридов 0,840 и 0,583% образуется при наличии в питательной среде солей магния и марганца. Оптимальный уровень рН для эффективной утилизации РВ питательной средыи выхода бактериальной биомассы составляет около 7, а температура в диапазоне 30–35 ?С.</p></abstract><trans-abstract xml:lang="en"><p>Leuconostoc mesenteroides is a well-known industrial producer of extracellular bacterial polysaccharide dextran. The main raw material for industrial cultivation of these microorganisms is molasses. Sucrose of molasses is the main source of carbon and energy for bacteria, as well as a precursor of dextran biosynthesis. It is known that the bacteria of the genus Leuconostoc are heterofermentative microorganisms and are capable to utilize various carbohydrates. The purpose of this study was to evaluate the of the corn biomass based mediumon the yield of bacterial biomass and the concentration of the extracellular polysaccharide.The hydrolyzate is a product of chemo-enzymatic treatment of plant lignocellulosic corn biomass and contains such monosaccharides as glucose, arabinose and xylose. The parameters of the composition of the medium were the concentration of hydrolyzate carbohydrates in the form of reducing substances (RS), the concentration of nitrogen (protein and non-protein), sucrose and the presence of minerals. The parameters of the cultivation process are pH and temperature. According to experimental data, the maximum yield of bacterial biomass (9.3 and 8.5%) were achieved on combined media with a sucrose content of 0.2% and a sodium salt (sodium phosphate), respectively. The highest specific growth rates of microorganisms (0.0840 and 0.0746) are demonstrated on a combined medium with a content of 0.15 and 0.3% inorganic nitrogen, respectively. The highest concentrations of extracellular polysaccharides (0.840 and 0.583%) are formed when magnesium and manganese salts are present in the nutrient medium. The optimal pH level for effective growth s is about 7, and the temperature in the range of 30-35 ° C.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>гидролизат лигноцеллюлозной биомассы</kwd><kwd>бактерии</kwd><kwd>внеклеточные полисахариды</kwd><kwd>питательная среда</kwd><kwd>культивирование</kwd><kwd>бактериальная биомасса</kwd></kwd-group><kwd-group xml:lang="en"><kwd>hydrolyzate of lignocellulosic biomass</kwd><kwd>bacteria</kwd><kwd>extracellular polysaccharides</kwd><kwd>nutrient medium</kwd><kwd>cultivation</kwd><kwd>bacterial biomass</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Mundt J.O. Lactic Acid Bacteria Associated with Raw Plant Food Material. // J. Milk Food Technol. 1970. №33. Р. 550–553</mixed-citation><mixed-citation xml:lang="en">Mundt J.O. 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