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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vguit</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Воронежского государственного университета инженерных технологий</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of the Voronezh State University of Engineering Technologies</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2226-910X</issn><issn pub-type="epub">2310-1202</issn><publisher><publisher-name>VSUET</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.20914/2310-1202-2021-1-258-262</article-id><article-id custom-type="elpub" pub-id-type="custom">vguit-2725</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Пищевая  биотехнология</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Food biotechnology</subject></subj-group></article-categories><title-group><article-title>Влияние закваски на аминокислотный состав кисломолочных продуктов</article-title><trans-title-group xml:lang="en"><trans-title>The effect of starter on amino acid composition of fermented milk</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4659-7047</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Занданова</surname><given-names>Т. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Zandanova</surname><given-names>T. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>к.т.н., доцент, кафедра технологии пищевых производств и индустрии питания, Сергеляхское ш. 3 км, 3, 677007, Якутск, Россия</p></bio><bio xml:lang="en"><p>Cand. Sci. (Engin.), associate professor, food technology and food industry department, Sergelyakhskoe highway 3km, building 3, Yakutsk,677007, Russia</p></bio><email xlink:type="simple">tuyana35@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2722-7825</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Иванова</surname><given-names>К. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Ivanova</surname><given-names>K. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>ст. препод, кафедра пищевых технологи и индустрии питания, Сергеляхское ш. 3 км, 3, 677007, Якутск, Россия</p></bio><bio xml:lang="en"><p>senior teacher, food technology and food industry department, Sergelyakhskoe highway 3km, building 3, Yakutsk,677007, Russia</p></bio><email xlink:type="simple">kiravlad85@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7300-0384</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мырьянова</surname><given-names>Т. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Myryuanova</surname><given-names>T. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>к.э.н., инженер, кафедра пищевых технологи и индустрии питания, Сергеляхское ш. 3 км, 3, 677007, Якутск, Россия</p></bio><bio xml:lang="en"><p>Cand. Sci. (Econ.), engineer, food technology and food industry department, Arctic State Agrotechnological University, Yakutsk,677007, Russia</p></bio><email xlink:type="simple">myryuanova@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Арктический государственный агротехнологический университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Arctic State Agrotechnological University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Арктический государственный агротехнологический университет</institution><country>Russian Federation</country></aff><aff xml:lang="en"><institution>Sergelyakhskoe highway 3km, building 3</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>28</day><month>04</month><year>2021</year></pub-date><volume>83</volume><issue>1</issue><fpage>258</fpage><lpage>262</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Занданова Т.Н., Иванова К.В., Мырьянова Т.П., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Занданова Т.Н., Иванова К.В., Мырьянова Т.П.</copyright-holder><copyright-holder xml:lang="en">Zandanova T.N., Ivanova K.V., Myryuanova T.P.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vestnik-vsuet.ru/vguit/article/view/2725">https://www.vestnik-vsuet.ru/vguit/article/view/2725</self-uri><abstract><p>Выполнены экспериментальные исследования аминокислотного состава коровьего обезжиренного молока, ферментированного кефирными грибками, естественной курунговой симбиотической закваской, бактериальным концентратом микробного консорциума (БКМК). Бактериальный концентрат микробного консорциума предназначен для приготовления кисломолочного напитка смешанного брожения – курунга. Для приготовления БКМК использован микробный консорциум, полученный путем автоселекции микрофлоры кефирной грибковой закваски и термофильных лактобактерий Lactobacillus bulgaricus и Lactobacillus acidophilum. Аминокислотный анализ образцов проводился методом ионной хроматографии с постколоночной дериватизацией аминокислот нингидрином в кислотном гидролизате образца на аминокислотном анализаторе «INGOSААА-400». В результате проведенных исследований установлено наибольшее количество незаменимых аминокислот 434 мг/г белка в кефире. В образцах, приготовленных на естественной закваске и бактериальном концентрате микробного консорциума, общее количество незаменимых аминокислот составило 401,84 и 403,8 мг/г белка соответственно. Для роста молочнокислых бактерий и дрожжей необходимы экзогенные аминокислоты. Различие общего количества незаменимых аминокислот в образцах, вероятно, связано с протеолитической активностью заквасок и ростовыми потребностями микроорганизмов. Оценку биологической ценности проводили по методике И.А. Рогова и Н.Н. Липатова по коэффициентам различий аминокислотного скора (КРАС) и биологической ценности (БЦ). Исследования аминокислотного скора показали, что белок в исследуемых образцах характеризуется полноценным составом, по БЦ образцы курунги превосходили кефир на 0,55-0,75%. Полученные результаты свидетельствуют о том, что по биохимической активности бактериальный концентрат микробного консорциума практически близок естественной закваске и позволяет получить курунгу идентичную по биологической ценности традиционному напитку.</p></abstract><trans-abstract xml:lang="en"><p>The experimental studies of the amino acid composition of the skimmed cow milk fermented with kefir corns, natural kurunga symbiotic starter and bacterial concentrate of the microbial consortium (BСMC) have been carried out. The bacterial concentrate of the microbial consortium is intended for the preparation of a fermented milk beverage of mixed fermentation – kurunga. The microbial consortium obtained by autoselection of population of kefir corn starter and thermophilic lactobacteriums Lactobacillus bulgaricus and Lactobacillus acidophilum has been used to prepare BCMC. The amino acid analysis of the samples has been carried out by IC method with postcolumn derivatization of amino acids ninhydrin in the acid hydrolyzate of the sample on INGOSAAA-400 amino acid analyzer. The most of essential amino acids has been found in kefir – 434 mg/g of protein. In the samples prepared with natural starter and bacterial concentrate, the total amount of essential amino acids has been 401.84 and 403.8 mg/g of protein, respectively. Exogenous amino acids are essential for the growth of lactics and yeast. The difference in the total amount of the essential amino acids in the samples, probably, caused by the proteolytic activity of the starters and the growth requirements of the microorganisms. The assessment of the bioavailability has been carried out by the method of I.A. Rogov and N.N. Lipatov according to the coefficients of differences between the amino-acid score (CDAS) and bioavailability. The amino-acid scoring studies have shown that the protein in the studied samples is characterized by a complete composition. According to bioavailability, the kurunga samples exceeded kefir by 0.55-0.75%. The obtained results indicate that according to the biochemical activity, the bacterial concentrate of the microbial consortium is practically identical to kurunga prepared with natural starter. It makes possible to obtain a product bioavailably identical to a traditional beverage.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>курунга</kwd><kwd>аминокислоты</kwd><kwd>автоселекция</kwd><kwd>биологическая ценность</kwd><kwd>молочнокислые бактерии</kwd><kwd>бактериальный концентрат</kwd><kwd>естественная закваска</kwd><kwd>аминокислотный скор</kwd><kwd>микробный консорциум</kwd><kwd>закваска</kwd></kwd-group><kwd-group xml:lang="en"><kwd>kurunga</kwd><kwd>amino acids</kwd><kwd>autoselection</kwd><kwd>bioavailability</kwd><kwd>lactics</kwd><kwd>bacterial concentrate</kwd><kwd>natural starter</kwd><kwd>amino acid score</kwd><kwd>microbial consortium</kwd><kwd>starter</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Арктический государственный агротехнологический университет</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Занданова Т.Н. Подбор питательной среды для получения бактериального концентрата микробного консорциума // Вестник ВСГУТУ. 2018. № 2. С. 67–72.</mixed-citation><mixed-citation xml:lang="en">Zandanova T.N. Selection of a nutrient medium for obtaining a bacterial concentrate of a microbial consortium. Vestnik VSGUTU. 2018. no. 2. pp. 67–72. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Надточий Л.А., Арсеньева Т.П. Букачакова Л.Ч. Витаминный состав и биологическая ценность алтайского кисломолочного напитка чеген // Научный журнал НИУ ИТМО. Серия «Процессы и аппараты пищевых производств». 2014. № 4. С. 1–6</mixed-citation><mixed-citation xml:lang="en">Nadtochy L.A., Arsenyeva T.P. Bukachakova L.Ch. Vitamin composition and biological value of the Altai fermented milk drink chegen. Scientific journal NRU ITMO. Series "Processes and Apparatus for Food Production". 2014. no. 4. pp. 1–6. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Нечаев А.П., Траубенберг С.Е., Кочеткова А.А. и др. Пищевая химия; 5е изд, испр и доп. СПБ.: ГИОРД, 2012. 672 с.</mixed-citation><mixed-citation xml:lang="en">Nechaev A.P., Traubenberg S.E., Kochetkova A.A. etc. Food chemistry; 5th ed., Revised and added. Saint Petersburg, GIORD, 2012. 672 p. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Лисин П.А., Канушина Ю.А. Аминокислотный состав творожного продукта // Молочная промышленность. 2011. № 11.С. 64–65</mixed-citation><mixed-citation xml:lang="en">Lisin P.A., Kanushina Yu.A. Amino acid composition of the curd product. Dairy industry. 2011. no. 11. pp. 64-65. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Cтоянова Л.Г., Устюгова Е.А., Нетрусов А.И. Антимикробные метаболиты молочнокислых бактерий: разнообразие и свойства (обзор) // Прикладная биохимия и микробиология. 2012. Т. 48. № 3. C.259–275.</mixed-citation><mixed-citation xml:lang="en">Stoyanova L.G., Ustyugova E.A., Netrusov A.I. Antimicrobial metabolites of lactic acid bacteria: diversity and properties (review). Applied biochemistry and microbiology. 2012. vol. 48. no. 3. pp. 259–275. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Крумликов В.Ю., Исамбетова Л.В. Исследование антибиотической резистентности, культуральных и морфологических свойств микроорганизмов, выделенных из национальных казахских напитков // Хранение и переработка сельхозсырья. 2016. №. 9. С. 31-35.</mixed-citation><mixed-citation xml:lang="en">Krumlikov V.Yu., Isambetova L.V. Investigation of antibiotic resistance, cultural and morphological properties of microorganisms isolated from national Kazakh drinks. Storage and processing of agricultural raw materials. 2016. no. 9. pp. 31-35. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Meister A. Biochemistry of the amino acids. Elsevier, 2012.</mixed-citation><mixed-citation xml:lang="en">Meister A. Biochemistry of the amino acids. Elsevier, 2012.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Wu G. Amino acids: biochemistry and nutrition. CRC Press, 2013.</mixed-citation><mixed-citation xml:lang="en">Wu G. Amino acids: biochemistry and nutrition. CRC Press, 2013.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Martinez B., Bottiger T., Schneider T., Rodriguez A. et al. Specific Interaction of the Unmodified Bacteriocin Lactococcin 972 with the Cell Wall Precursor Lipid II // Appl. Environ. Microbiol.2009. V. 74. P. 4666–4670.</mixed-citation><mixed-citation xml:lang="en">Martinez B., Bottiger T., Schneider T., Rodriguez A. et al. Specific Interaction of the Unmodified Bacteriocin Lactococcin 972 with the Cell Wall Precursor Lipid II. Appl. Environ. Microbiol.2009. vol. 74. pp. 4666–4670.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Barrett G. Chemistry and biochemistry of the amino acids. Springer Science &amp; Business Media, 2012.</mixed-citation><mixed-citation xml:lang="en">Barrett G. Chemistry and biochemistry of the amino acids. Springer Science &amp; Business Media, 2012.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Stadie J., Matthias Gulitz A., Ehrmann Rudi A., Vogel F. Metabolic activity and symbiotic interactions of lactic acid bacteria and yeasts isolated from water kefir // Food Microbiology. 2013. V. 35. № 2. P. 92–98. doi: 10.1016/j.fm.2013.03.009</mixed-citation><mixed-citation xml:lang="en">Stadie J., Matthias Gulitz A., Ehrmann Rudi A., Vogel F. Metabolic activity and symbiotic interactions of lactic acid bacteria and yeasts isolated from water kefir. Food Microbiology. 2013. vol. 35. no. 2. pp. 92–98. doi: 10.1016/j.fm.2013.03.009</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Hati S., Patel N., Sakure A., Mandal S. Influence of whey protein concentrate on the production of antibacterial peptides derived from fermented milk by lactic acid bacteria // International Journal of Peptide Research and Therapeutics. 2018. V. 24. №. 1. P. 87-98. doi: 10.1007/s10989-017-9596-2</mixed-citation><mixed-citation xml:lang="en">Hati S., Patel N., Sakure A., Mandal S. Influence of whey protein concentrate on the production of antibacterial peptides derived from fermented milk by lactic acid bacteria. International Journal of Peptide Research and Therapeutics. 2018. vol. 24. no. 1. pp. 87-98. doi: 10.1007/s10989-017-9596-2</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Rama G.R. Kuhn D., Beux S., Maciel M.J. et al. Potential applications of dairy whey for the production of lactic acid bacteria cultures // International Dairy Journal. 2019. V. 98. P. 25-37. doi: 10.1016/j.idairyj.2019.06.012</mixed-citation><mixed-citation xml:lang="en">Rama G.R. Kuhn D., Beux S., Maciel M.J. et al. Potential applications of dairy whey for the production of lactic acid bacteria cultures. International Dairy Journal. 2019. vol. 98. pp. 25-37. doi: 10.1016/j.idairyj.2019.06.012</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Mohammadi R., Sohrabvandi S., Mohammad Mortazavian A. The starter culture characteristics of probiotic microorganisms in fermented milks // Engineering in Life Sciences. 2012. V. 12. №. 4. P. 399-409. doi: 10.1002/elsc.201100125</mixed-citation><mixed-citation xml:lang="en">Mohammadi R., Sohrabvandi S., Mohammad Mortazavian A. The starter culture characteristics of probiotic microorganisms in fermented milks. Engineering in Life Sciences. 2012. vol. 12. no. 4. pp. 399-409. doi: 10.1002/elsc.201100125</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Ozcan T., Sahin S., Akpinar?Bayizit A., Yilmaz?Ersan L. Assessment of antioxidant capacity by method comparison and amino acid characterisation in buffalo milk kefir // International Journal of Dairy Technology. 2019. V. 72. №. 1. P. 65-73. doi: 10.1111/1471-0307.12560</mixed-citation><mixed-citation xml:lang="en">Ozcan T., Sahin S., Akpinar?Bayizit A., Yilmaz?Ersan L. Assessment of antioxidant capacity by method comparison and amino acid characterisation in buffalo milk kefir. International Journal of Dairy Technology. 2019. vol. 72. no. 1. pp. 65-73. doi: 10.1111/1471-0307.12560</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Akabanda F., Owusu-Kwarteng J., Tano-Debrah K., Parkouda C. et al. The use of lactic acid bacteria starter culture in the production of Nunu, a spontaneously fermented milk product in Ghana // International journal of food science. 2014. V. 2014. doi: 10.1155/2014/721067</mixed-citation><mixed-citation xml:lang="en">Akabanda F., Owusu-Kwarteng J., Tano-Debrah K., Parkouda C. et al. The use of lactic acid bacteria starter culture in the production of Nunu, a spontaneously fermented milk product in Ghana. International journal of food science. 2014. vol. 2014. doi: 10.1155/2014/721067</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Ili?i? M.D., Milanovi? S.D., Cari? M.?., Vuki? V.R. et al. The effect of transglutaminase on rheology and texture of fermented milk products // Journal of Texture Studies. 2013. V. 44. №. 2. P. 160-168. doi: 10.1111/jtxs.12008</mixed-citation><mixed-citation xml:lang="en">Ili?i? M.D., Milanovi? S.D., Cari? M.?., Vuki? V.R. et al. The effect of transglutaminase on rheology and texture of fermented milk products. Journal of Texture Studies. 2013. vol. 44. no. 2. pp. 160-168. doi: 10.1111/jtxs.12008</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Gul O., Mortas M., Atalar I., Dervisoglu M. et al. Manufacture and characterization of kefir made from cow and buffalo milk, using kefir grain and starter culture // Journal of dairy science. 2015. V. 98. №. 3. P. 1517-1525. doi: 10.3168/jds.2014-8755</mixed-citation><mixed-citation xml:lang="en">Gul O., Mortas M., Atalar I., Dervisoglu M. et al. Manufacture and characterization of kefir made from cow and buffalo milk, using kefir grain and starter culture. Journal of dairy science. 2015. vol. 98. no. 3. pp. 1517-1525. doi: 10.3168/jds.2014-8755</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Kakimov A., Kakimova Z., Mirasheva G., Bepeyeva A. et al. Amino acid composition of sour-milk drink with encapsulated probiotics // Annual Research &amp; Review in Biology. 2017. P. 1-7. doi: 10.9734/ARRB/2017/36079</mixed-citation><mixed-citation xml:lang="en">Kakimov A., Kakimova Z., Mirasheva G., Bepeyeva A. et al. Amino acid composition of sour-milk drink with encapsulated probiotics. Annual Research &amp; Review in Biology. 2017. pp. 1-7. doi: 10.9734/ARRB/2017/36079</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Widyastuti Y., Febrisiantosa A.The role of lactic acid bacteria in milk fermentation // Food and Nutrition Sciences. 2014. V. 2014. doi: 10.4236/fns.2014.54051</mixed-citation><mixed-citation xml:lang="en">Widyastuti Y., Febrisiantosa A.The role of lactic acid bacteria in milk fermentation. Food and Nutrition Sciences. 2014. vol. 2014. doi: 10.4236/fns.2014.54051</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
