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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vguit</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Воронежского государственного университета инженерных технологий</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of the Voronezh State University of Engineering Technologies</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2226-910X</issn><issn pub-type="epub">2310-1202</issn><publisher><publisher-name>VSUET</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.20914/2310-1202-2024-2-255-261</article-id><article-id custom-type="elpub" pub-id-type="custom">vguit-3386</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Пищевая  биотехнология</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Food biotechnology</subject></subj-group></article-categories><title-group><article-title>Влияние ультразвуковой обработки на экстрагируемость белков при комплексной переработке семян подсолнечника</article-title><trans-title-group xml:lang="en"><trans-title>Effects of ultrasonic treatment on protein extractability during complex processing of sunflower seeds</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шагинова</surname><given-names>Л. О.</given-names></name><name name-style="western" xml:lang="en"><surname>Shaginova</surname><given-names>L. O.</given-names></name></name-alternatives><bio xml:lang="ru"><p>младший научный сотрудник, отдел производства пищевых растительных белков и биотехнологии, ул. Черняховского, 10, г. Санкт-Петербург, 191119, Россия</p></bio><bio xml:lang="en"><p>junior researcher, production of food vegetable proteins and biotechnology department, Chernnyakhovskiy st., 10, St. Petersburg, 191119, Russia</p></bio><email xlink:type="simple">noreplay@elpub.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Забодалова</surname><given-names>Л. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Zabodalova</surname><given-names>L. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>д.т.н, доцент, факультет биотехнологий, Кронверкский пр., 49, г. Санкт-Петербург, 197101, Россия</p></bio><email xlink:type="simple">noreplay@elpub.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Демьяненко</surname><given-names>Т. Ф.</given-names></name><name name-style="western" xml:lang="en"><surname>Demianenko</surname><given-names>T. F.</given-names></name></name-alternatives><bio xml:lang="ru"><p>д.т.н, доцент, факультет биотехнологий, Кронверкский пр., 49, г. Санкт-Петербург, 197101, Россия</p></bio><email xlink:type="simple">noreplay@elpub.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Доморощенкова</surname><given-names>М. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Domoroshchenkova</surname><given-names>M. L.</given-names></name></name-alternatives><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Крылова</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Krylova</surname><given-names>I. V.</given-names></name></name-alternatives><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ВНИИЖиров</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russian Research Institute of Fats</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Университет ИТМО</institution><country>Russian Federation</country></aff><aff xml:lang="en"><institution>ITMO University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>ВНИИЖиров</institution><country>Russian Federation</country></aff><aff xml:lang="en"><institution>ARSRIF</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>16</day><month>05</month><year>2024</year></pub-date><volume>86</volume><issue>2</issue><fpage>255</fpage><lpage>261</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Шагинова Л.О., Забодалова Л.А., Демьяненко Т.Ф., Доморощенкова М.Л., Крылова И.В., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Шагинова Л.О., Забодалова Л.А., Демьяненко Т.Ф., Доморощенкова М.Л., Крылова И.В.</copyright-holder><copyright-holder xml:lang="en">Shaginova L.O., Zabodalova L.A., Demianenko T.F., Domoroshchenkova M.L., Krylova I.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vestnik-vsuet.ru/vguit/article/view/3386">https://www.vestnik-vsuet.ru/vguit/article/view/3386</self-uri><abstract><p>В данном исследовании в качестве сырья для получения белковых препаратов подсолнечника использовали шрот семян подсолнечника, полученный на шнековом прессе путем холодного прессования лущеных семян подсолнечника кондитерского типа. Ультразвуковую обработку безфенольного жмыха применяли для улучшения экстрагируемости белков после удаления фенольных соединений путем обработки обрушенного подсолнечного жмыха водным раствором этилового спирта. Озвучивание осуществлялось генератором на частоте ультразвука 22,00 ± 1,65 кГц с длительностью ультразвуковой обработки от 5 до 15 минут. Экстракцию белков проводили в слабощелочной среде 0,1 % раствором бикарбоната натрия с гидромодулем 1:5 и температурой 45±2°С. Содержание белка и сухого вещества в белковых экстрактах пропорционально увеличивалось через 5–12 мин. ультразвуковая обработка с образованием гомогенных белковых экстрактов, устойчивых к разделению твердых фаз и характеризующихся более светлой окраской по сравнению с контрольным образцом, не подвергнутым ультразвуковой обработке. Однако уже через 15 минут ультразвуковой обработки белковые экстракты теряли однородность с выпадением в осадок твердой фракции. Исследования показали, что применение ультразвуковой обработки перед экстракцией белка приводило к увеличению содержания сухого вещества и сырого белка в экстрактах белков подсолнечника соответственно продолжительности обработки ультразвуком. Этот эффект может быть полезен для увеличения выхода белка в процессах производства светлых белковых концентратов и изолятов подсолнечника, а также для производства функциональных белковых напитков из семян подсолнечника. Кроме того, показано, что обработка ультразвуком растворов белков подсолнечника, полученных после экстракции белковых веществ 0,1 % раствором NaНСО3 и удаления нерастворимого остатка, существенно улучшала их седиментационную устойчивость и приводила к более светлой окраске полученных белковых растворов по сравнению с традиционной обработкой высокоскоростной диспергатор. Такая обработка может быть использована в процессах производства аналогов молочного молока из семян подсолнечника</p></abstract><trans-abstract xml:lang="en"><p>In this study sunflower seed cake produced on a screw press by cold pressing of dehulled sunflower seeds of confectionery-type sunflower was used as a raw material for obtaining of sunflower protein preparations. Ultrasonic treatment of the phenol-free cake was applied for improvement of extractability of proteins after the removal of phenolic compounds by treatment of dehulled sunflower press cake with an aqueous ethyl alcohol solution. Sonification was accomplished by the generator at ultrasound frequency of 22.00 ± 1.65 kНz with a duration of ultrasonic treatment from 5 to 15 minutes. Extraction of proteins was carried out in a mild alkaline medium with 0.1 % sodium bicarbonate solution with a hydro module of 1: 5 and temperature of 45 ± 2° C. Protein and dry matter content of protein extracts proportionally increased after 5–12 minutes of ultrasonic treatment with the formation of homogeneous protein extracts stable to solid phase separation and characterized by a lighter colour in comparison with the control sample not subjected to ultrasonic processing. However, after 15 minutes of ultrasonic treatment protein extracts lost uniformity with sedimentation of a solid fraction. Studies demonstrated that application of ultrasonic treatment prior protein extraction resulted in a growth of dry matter and crude protein content of sunflower protein extracts correspondently to the duration of sonification. This effect might be beneficial for an increase of protein yields in processes of manufacturing of light-coloured sunflower protein concentrates and isolates and for manufacturing of functional protein drinks from sunflower seeds. Additionally, it has been shown that sonication of sunflower protein solutions obtained after extraction of protein substances with 0.1 % NаНСО3 solution and removal of insoluble residue significantly improved their sedimentation stability and resulted in a lighter colour of resulted protein solutions in comparison with a conventional treatment by a high-speed disperser. Such treatment might be used in processes of manufacturing of dairy milk analogues from sunflower seeds. </p></trans-abstract><kwd-group xml:lang="ru"><kwd>семена подсолнечника</kwd><kwd>белок подсолнечника</kwd><kwd>экстракция белка</kwd><kwd>фенольные соединения</kwd><kwd>ультразвуковая обработка</kwd></kwd-group><kwd-group xml:lang="en"><kwd>sunflower seeds</kwd><kwd>sunflower protein</kwd><kwd>protein extraction</kwd><kwd>phenolic compounds</kwd><kwd>ultrasonic treatment</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Aiking H., de Boer J. The next protein transition. Trends in Food Science and Technology. 2020. vol. 105. pp. 515–522.</mixed-citation><mixed-citation xml:lang="en">Aiking H., de Boer J. The next protein transition. Trends in Food Science and Technology. 2020. vol. 105. pp. 515–522.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Agroinvestor. Available at: https://www.agroinvestor.ru/markets/news/35506alternativnye-moloko-i-myaso-mogut-zanyat11rynka-belkov-k2035godu/</mixed-citation><mixed-citation xml:lang="en">Agroinvestor. Available at: https://www.agroinvestor.ru/markets/news/35506alternativnye-moloko-i-myaso-mogut-zanyat11rynka-belkov-k2035godu/</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Taha F.S., Abbasy M., El Nockrashy A.S., Shoeb Z.E. Countercurrent extraction and isoelectric precipitation of sunflower seed protein isolates. Journal of the Science of Food and Agriculture. 1981. vol. 32. no. 2. pp. 166–174.</mixed-citation><mixed-citation xml:lang="en">Taha F.S., Abbasy M., El Nockrashy A.S., Shoeb Z.E. Countercurrent extraction and isoelectric precipitation of sunflower seed protein isolates. Journal of the Science of Food and Agriculture. 1981. vol. 32. no. 2. pp. 166–174.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Parrado J., Bautista J., Machado A. Production of soluble enzymic protein hydrolyzate from industrially defatted nondehulled sunflower meal. Journal of agricultural and food chemistry. 1991. vol. 39. no. 3. pp. 447–450.</mixed-citation><mixed-citation xml:lang="en">Parrado J., Bautista J., Machado A. Production of soluble enzymic protein hydrolyzate from industrially defatted nondehulled sunflower meal. Journal of agricultural and food chemistry. 1991. vol. 39. no. 3. pp. 447–450.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">González-Pérez S., Merck K.B., Vereijken J.M. et al. Isolation and characterization of undenatured chlorogenic acid free sunflower (Helianthus annuus) proteins. Journal of Agricultural Food Chemistry. 2002. vol. 50. no. 6. pp. 1713–1719.</mixed-citation><mixed-citation xml:lang="en">González-Pérez S., Merck K.B., Vereijken J.M. et al. Isolation and characterization of undenatured chlorogenic acid free sunflower (Helianthus annuus) proteins. Journal of Agricultural Food Chemistry. 2002. vol. 50. no. 6. pp. 1713–1719.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Ivanova P., Chalova V., Koleva L., Pishtiyski I. et al. Optimization of protein extraction from sunflower meal produced in Bulgaria. Bulg. J. Agric. Sci. 2012. vol. 18. pp. 153–160.</mixed-citation><mixed-citation xml:lang="en">Ivanova P., Chalova V., Koleva L., Pishtiyski I. et al. Optimization of protein extraction from sunflower meal produced in Bulgaria. Bulg. J. Agric. Sci. 2012. vol. 18. pp. 153–160.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Shirokoryadova O.V. et al. Sunflower meal is an economically promising raw material for the production of food protein and carbohydrate products Izvestiya vuzov. Food technology. 2009. vol. 5. no. 6. pp. 45–48.</mixed-citation><mixed-citation xml:lang="en">Shirokoryadova O.V. et al. Sunflower meal is an economically promising raw material for the production of food protein and carbohydrate products Izvestiya vuzov. Food technology. 2009. vol. 5. no. 6. pp. 45–48.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Pickardt C., Hager T., Eisner P., Carle R. et al. Isoelectric protein precipitation from mild-acidic extracts of de-oiled sunflower (Helianthus annuus L.) press cake. Eur Food Res Technol. 2011. vol. 233. pp. 31–44.</mixed-citation><mixed-citation xml:lang="en">Pickardt C., Hager T., Eisner P., Carle R. et al. Isoelectric protein precipitation from mild-acidic extracts of de-oiled sunflower (Helianthus annuus L.) press cake. Eur Food Res Technol. 2011. vol. 233. pp. 31–44.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Voichenko O.N. et al. Evaluation of sunflower seed processing products as alternative sources of dietary protein. Izvestiya vuzov Food technology. 2013. vol. 4. no. 334. pp. 88–90.</mixed-citation><mixed-citation xml:lang="en">Voichenko O.N. et al. Evaluation of sunflower seed processing products as alternative sources of dietary protein. Izvestiya vuzov Food technology. 2013. vol. 4. no. 334. pp. 88–90.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Shchekoldina T.V. Technology for production of protein-containing raw materials from the products of sunflower seeds. Polythematic online scientific journal of Kuban State Agrarian University. 2015. vol. 109. no. 05. pp. 360–378.</mixed-citation><mixed-citation xml:lang="en">Shchekoldina T.V. Technology for production of protein-containing raw materials from the products of sunflower seeds. Polythematic online scientific journal of Kuban State Agrarian University. 2015. vol. 109. no. 05. pp. 360–378.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Domoroshchenkova M.L., Demianenko T.F., Krylova I.V. Prospects for obtaining proteins from sunflower meal. Oils and Fats. 2016. vol. 5–6. pp. 22–23.</mixed-citation><mixed-citation xml:lang="en">Domoroshchenkova M.L., Demianenko T.F., Krylova I.V. Prospects for obtaining proteins from sunflower meal. Oils and Fats. 2016. vol. 5–6. pp. 22–23.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Ovsiannikova O.V., Frantseva T.P. Development of technology for obtaining food protein products from sunflower seeds. Saint-Petersburg, Lan, 2017.</mixed-citation><mixed-citation xml:lang="en">Ovsiannikova O.V., Frantseva T.P. Development of technology for obtaining food protein products from sunflower seeds. Saint-Petersburg, Lan, 2017.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Cater C.M., Gheyasuddin S., Mattil K.F. The effect of chlorogenic, quinic and caffeic acids on the solubility and color of protein isolates, especially from sunflower seed. Cereal Chem. 1972. vol. 49. pp. 508–514.</mixed-citation><mixed-citation xml:lang="en">Cater C.M., Gheyasuddin S., Mattil K.F. The effect of chlorogenic, quinic and caffeic acids on the solubility and color of protein isolates, especially from sunflower seed. Cereal Chem. 1972. vol. 49. pp. 508–514.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Vedernikova E.I. Phenolic compounds of sunflower protein isolates. Applied Biochemistry and Microbiology. 1974. vol. 10. no. 06. pp. 897–905.</mixed-citation><mixed-citation xml:lang="en">Vedernikova E.I. Phenolic compounds of sunflower protein isolates. Applied Biochemistry and Microbiology. 1974. vol. 10. no. 06. pp. 897–905.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Sabir M.A., Sosulski F.W., Finlayson A.J. Chlorogenic acid-protein interactions in Sunflower. J. Agric. Food Chem. 1974. vol. 22. no. 4. pp. 575–578.</mixed-citation><mixed-citation xml:lang="en">Sabir M.A., Sosulski F.W., Finlayson A.J. Chlorogenic acid-protein interactions in Sunflower. J. Agric. Food Chem. 1974. vol. 22. no. 4. pp. 575–578.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Leung J., Fenton T.W., Clandinin D.R. Phenolic components of sunflower flour. J. Food Sci. 1981. vol. 46. no. 5. pp. 1386–1388.</mixed-citation><mixed-citation xml:lang="en">Leung J., Fenton T.W., Clandinin D.R. Phenolic components of sunflower flour. J. Food Sci. 1981. vol. 46. no. 5. pp. 1386–1388.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Sripad G., Prakash V., Narasinga Rao M.S. Extractability of polyphenols of sunflower seed in various solvents. J. Biosci. 1982. vol. 4. no. 2. pp. 145–152.</mixed-citation><mixed-citation xml:lang="en">Sripad G., Prakash V., Narasinga Rao M.S. Extractability of polyphenols of sunflower seed in various solvents. J. Biosci. 1982. vol. 4. no. 2. pp. 145–152.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Weisz G.M., Kammerer D.R., Carle R. Identification and quantification of phenolic compounds from sunflower (Helianthus annuus L.) kernels and shells by HPLCDAD/ESI-MSn. Food Chem. 2009. vol. 115. no. 2. pp. 758–765.</mixed-citation><mixed-citation xml:lang="en">Weisz G.M., Kammerer D.R., Carle R. Identification and quantification of phenolic compounds from sunflower (Helianthus annuus L.) kernels and shells by HPLCDAD/ESI-MSn. Food Chem. 2009. vol. 115. no. 2. pp. 758–765.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Shaginova L.O., Krylova I.V., Demianenko T.F., Domoroshchenkova M.L. Research of the process of obtaining protein preparation from sunflower seeds for food industry. New technologies. 2021. vol. 17. no. 3. pp. 41–50.</mixed-citation><mixed-citation xml:lang="en">Shaginova L.O., Krylova I.V., Demianenko T.F., Domoroshchenkova M.L. Research of the process of obtaining protein preparation from sunflower seeds for food industry. New technologies. 2021. vol. 17. no. 3. pp. 41–50.</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Pojić M., Mišan A., Tiwari B. Eco-innovative technologies for extraction of proteins for human consumption from renewable protein sources of plant origin. Trends in Food Science and Technology. 2018. vol. 75. pp. 93–104.</mixed-citation><mixed-citation xml:lang="en">Pojić M., Mišan A., Tiwari B. Eco-innovative technologies for extraction of proteins for human consumption from renewable protein sources of plant origin. Trends in Food Science and Technology. 2018. vol. 75. pp. 93–104.</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Rahman M.M., Lamsal B.P. Ultrasound-assisted extraction andmodification of plant-based proteins: Impact onphysicochemical, functional, and nutritionalproperties. Compr Rev Food Sci Food Saf. 2021. vol. 20. pp. 1457–1480.</mixed-citation><mixed-citation xml:lang="en">Rahman M.M., Lamsal B.P. Ultrasound-assisted extraction andmodification of plant-based proteins: Impact onphysicochemical, functional, and nutritionalproperties. Compr Rev Food Sci Food Saf. 2021. vol. 20. pp. 1457–1480.</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru"></mixed-citation><mixed-citation xml:lang="en"></mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
