vguitВестник Воронежского государственного университета инженерных технологийProceedings of the Voronezh State University of Engineering Technologies2226-910X2310-1202VSUET10.20914/2310-1202-2024-3-166-174vguit-3522Research ArticleПищевая биотехнологияFood biotechnologyФизико-химические свойства и липидосвязывающая способность бактериальной целлюлозы, синтезированной на молочной сывороткеPhysico-chemical properties and lipid binding capacity of bacterial cellulose synthesized on wheyhttps://orcid.org/0000-0001-8495-3869ПогореловаН. А.PogorelovaN. A.

к.б.н., доцент, кафедры продуктов питания и пищевой биотехнологии, ул. Институтская площадь, 1, г. Омск, 644008, Россия

Cand. Sci. (Biol.), associate professor, nutrition and food biotechnology departments, 1 Institutskaya Ploshchad str., Omsk, 644008, Russia

na.pogorelova@omgau.orghttps://orcid.org/0000-0002-1614-5090СарницкаяН. А.SarnitskayaN. A.

аспирант, кафедры продуктов питания и пищевой биотехнологии, ул. Институтская площадь, 1, г. Омск, 644008, Россия

graduate student, nutrition and food biotechnology departments, 1 Institutskaya Ploshchad str., Omsk, 644008, Russia

na.sarnitskaya@omgau.orghttps://orcid.org/0000-0002-8817-1466ПолянскийК. К.PolyanskyK. K.

д.т.н., профессор, кафедра управления социально-экономическими системами и бизнес-процессами, ул. Карла Маркса,67А, Воронеж, 394030, Россия

Dr. Sci. (Engin.), professor, management of socio-economic systems and business processes department, Karla Marksa, 67A, Voronezh, 394030, Russia

mto.vrn@mail.ruhttps://orcid.org/0000-0002-9726-9262ДерканосоваА. А.DerkanosovaA. A.

д.т.н., профессор, кафедра сервиса и ресторанного бизнеса, пр-т Революций, 19, г. Воронеж, 394036, Россия

Dr. Sci.(Engin), professor, service and restaurant business department, Revolution Av.,19 Voronezh, 394036

aa-derk@ya.ruhttps://orcid.org/0000-0003-3537-8081КоноваловС. А.KonovalovS. A.

к.т.н., доцент, кафедра продуктов питания и пищевой биотехнологии, г. Омск, 644008 Россия

Cand. Sci (Engin.), associate professor, food and food biotechnology department, Institutskaya square, 1, Omsk, 644008, Russia

sa.konovalov@omgau.orgОмский государственный аграрный университет имени П.А. СтолыпинаOmsk State Agrarian University named after P.A. StolypinВоронежский филиал Российского экономического университета имени Г.В. ПлехановаVoronezh branch of the Russian Economic University named after G.V. PlekhanovВоронежский государственный университет инженерных технологийVoronezh State University of Engineering TechnologiesОмский государственный аграрный университет имени П.А. СтолыпинаOmsk State Agrarian University named after. P.A. Stolypin202411102024863166174Copyright © Погорелова Н.А., Сарницкая Н.А., Полянский К.К., Дерканосова А.А., Коновалов С.А., 20242024Погорелова Н.А., Сарницкая Н.А., Полянский К.К., Дерканосова А.А., Коновалов С.А.Pogorelova N.A., Sarnitskaya N.A., Polyansky K.K., Derkanosova A.A., Konovalov S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vestnik-vsuet.ru/vguit/article/view/3522

Бактериальная целлюлоза (БЦ) имеет значительный потенциал применений в пищевой промышленности, в результате ее способности стабилизировать пищевые дисперсные системы. В данной работе исследовали эффективность биосинтеза БЦ на отходах молочной промышленности, изучены параметры процесса: количество субстрата, титруемая кислотность, масса синтезируемой целлюлозы. Определены физико-химические свойства и липидосвязывающая способность полученных гель пленок и дегидратированных образцов полисахарида. Бактериальную целлюлозу получали в статических условиях, на жидкой питательной среде – молочной сыворотке. Предварительно лактозу молочной сыворотки гидролизовали ферментным препаратом LACTA FREE до количества глюкозы 1.93%. Масса продуцируемой целлюлозы в статических условиях симбиотической консорциумом Medusomyces gisevii составила 3.84 г/л в течение 21-х суток при Т=31°С. Отмечена корреляция низких количеств глюкозы культуральной жидкости на 6-е сутки биосинтеза со снижением значений титруемой кислотности. Степень конверсии глюкозы ферментированной молочной сыворотки на массу продуцируемой бактериальной целлюлозы составила 22.6%. Максимальная скорость биосинтеза 0.181 г СВ/л×сут установлена на 15-е сутки процесса. Определена водоудерживающая способность синтезированных гель пленок бактериальной целлюлозы – 82.35 ± 0.63 г/г. Лиофилизированные образцы бактериальной целлюлозы характеризовались большей водопоглащающей способностью (22.44 г/г) в сравнении с дегидратированными при Т=50°С образцами (5.42 г/г). Установлено, что регидратированные образцы лиофилизированной целлюлозы обладали в 3.43 раза большим показателем отношения количества свободной и связанной влаги в сравнении с высушиваемыми образцами БЦ. Определена насыпная плотность дезагрегированной целлюлозы: лиофилизированной - 20 кг/м3, термически высушенной - 170 кг/м3. В работе дана сравнительная характеристика липидосвязывающей способности БЦ и хитозана. Определены микробиологические показатели дезагрегированной бактериальной целлюлозы.

Bacterial cellulose (BC) has significant potential for applications in the food industry due to its ability to stabilize food dispersion systems. In this work, the efficiency of BC biosynthesis on dairy industry waste was investigated, and these process parameters were studied: amount of substrate, titratable acidity, mass of synthesized cellulose. The physico-chemical properties and lipid binding capacity of the obtained gel films and dehydrated polysaccharide samples were determined. Bacterial cellulose was obtained under static conditions, in a liquid nutrient medium – whey. Lactose from whey was preliminarily hydrolysed with the enzyme preparation LACTA FREE to a glucose content of 1.93%. The mass of cellulose produced under static conditions by the symbiotic consortium Medusomyces gisevii was 3.84 g/l over 21 days at T=31°C. A correlation was noted between low amounts of glucose in the culture fluid on the 6th day of biosynthesis and a decrease in titratable acidity. The degree of conversion of glucose from fermented whey to the mass of produced bacterial cellulose was 22.6%. The maximum biosynthesis rate of 0.181 gL-1×day-1 (dry weight) was established on the 15th day of the process. The water retention capacity of the synthesized bacterial cellulose gel films was determined to be 82.35 ± 0.63 g/g. Lyophilized samples of bacterial cellulose were characterized by a higher water absorption capacity (22.44 g/g) compared to samples dehydrated at T=50°C (5.42 g/g). It was found that rehydrated samples of lyophilized cellulose had a 3.43 times higher ratio of free and bound moisture compared to dried BC samples. The bulk density of disaggregated cellulose was determined: lyophilized - 20 kg/m3, thermally dried - 170 kg/m3. The work provides a comparative analysis of the lipid binding capacity of BC and chitosan. Microbiological parameters of disaggregated bacterial cellulose were determined.

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The authors declare that there are no conflicts of interest present.