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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vguit</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Воронежского государственного университета инженерных технологий</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of the Voronezh State University of Engineering Technologies</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2226-910X</issn><issn pub-type="epub">2310-1202</issn><publisher><publisher-name>VSUET</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.20914/2310-1202-2025-4-86-92</article-id><article-id custom-type="elpub" pub-id-type="custom">vguit-3721</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Пищевые системы</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Food systems</subject></subj-group></article-categories><title-group><article-title>Эффективность комбинированного ферментативного гидролиза для получения высокобелкового гидролизата из гороха</article-title><trans-title-group xml:lang="en"><trans-title>Efficiency of combined enzymatic hydrolysis for producing high-protein hydrolysate from pea</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1285-4797</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бикбулатов</surname><given-names>П. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Bikbulatov</surname><given-names>P. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>к.т.н., доцент, кафедра технологии питания, ул. 8 Марта/Народной Воли, 62/45, г. Екатеринбург, 620144, Россия</p></bio><bio xml:lang="en"><p>Cand. Sci. (Engin.), assistant professor, food technology department, 8 Marta str./Narodnaya Volya, 62/45, Yekaterinburg, 620144, Russia</p></bio><email xlink:type="simple">bikbulatovpavel@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9831-3635</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Панкратьева</surname><given-names>Н. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Pankratyeva</surname><given-names>N. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>д.х.н., профессор, кафедра технологии питания, ул. 8 Марта/Народной Воли, 62/45, г. Екатеринбург, 620144, Россия</p></bio><bio xml:lang="en"><p>Dr. Sci. (Chem.), professor, food technology department, 8 Marta str./Narodnaya Volya, 62/45, Yekaterinburg, 620144, Russia</p></bio><email xlink:type="simple">nata-pankratieva@ya.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7039-4047</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чугунова</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Chugunova</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>к.э.н., инженер, кафедра технологии питания, ул. 8 Марта/Народной Воли, 62/45, г. Екатеринбург, 620144, Россия</p></bio><bio xml:lang="en"><p>Cand. Sci. (Econ.), engineer, food technology department, 8 Marta str./Narodnaya Volya, 62/45, Yekaterinburg, 620144, Russia</p></bio><email xlink:type="simple">chugun.ova@ya.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8396-0431</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Донскова</surname><given-names>Л. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Donskova</surname><given-names>L. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>, кафедра технологии питания, ул. 8 Марта/Народной Воли, 62/45, г. Екатеринбург, 620144, Россия</p></bio><bio xml:lang="en"><p>, food technology department, 8 Marta str./Narodnaya Volya, 62/45, Yekaterinburg, 620144, Russia</p></bio><email xlink:type="simple">cafedra@list.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Уральский государственный экономический университет</institution></aff><aff xml:lang="en"><institution>Ural State University of Economics</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>30</day><month>12</month><year>2025</year></pub-date><volume>87</volume><issue>4</issue><fpage>86</fpage><lpage>92</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Бикбулатов П.С., Панкратьева Н.А., Чугунова О.В., Донскова Л.А., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Бикбулатов П.С., Панкратьева Н.А., Чугунова О.В., Донскова Л.А.</copyright-holder><copyright-holder xml:lang="en">Bikbulatov P.S., Pankratyeva N.A., Chugunova O.V., Donskova L.A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vestnik-vsuet.ru/vguit/article/view/3721">https://www.vestnik-vsuet.ru/vguit/article/view/3721</self-uri><abstract><p>Исследована эффективность ферментативных и комбинированных методов выделения и модификации растительного белка из гороха для получения высокобелкового гидролизата с улучшенными функциональными свойствами. Цель работы состояла в сравнительной оценке различных схем гидролиза и обосновании оптимального технологического решения. Объектом исследования служили гидролизаты белка гороха, полученные без обработки, с применением протеазы и лактата кальция, а также при последовательном использовании амилазы и протеазы. Установлено, что контрольный образец характеризуется низкой массовой долей белка 27.5% и отсутствием выраженного гидролиза. Использование протеазы в сочетании с лактатом кальция обеспечивает повышение содержания белка до 55.8% и степень гидролиза 9.77%, что связано с расщеплением глобулярных белков и улучшением их растворимости. Наибольший эффект достигнут при комбинированном ферментативном гидролизе с предварительным действием амилазы, при котором массовая доля белка возрастает до 70.5%, содержание аминного азота достигает 698.66 мг на 100 г, а степень гидролиза составляет 13.41%. Снижение выхода сухого остатка с 28.5 до 16.8 г подтверждает эффективное удаление небелковых компонентов, прежде всего крахмала и клетчатки. Показано, что применение амилазы снижает вязкость системы и повышает доступность белкового субстрата для протеазы, а лактат кальция интенсифицирует коагуляцию и отделение белковой фазы. Сделан вывод о целесообразности использования комбинированного ферментативного гидролиза для получения высокобелковых растительных ингредиентов с повышенной биодоступностью, которые рекомендованы для функциональных и специализированных пищевых продуктов.</p></abstract><trans-abstract xml:lang="en"><p>The study evaluates the efficiency of enzymatic and combined approaches for the extraction and modification of pea protein aimed at producing a high protein hydrolysate with improved functional properties. The objective was to compare different hydrolysis schemes and to justify an optimal technological solution. Pea protein hydrolysates obtained without treatment, with protease and calcium lactate, and with sequential application of amylase and protease were investigated. The control sample showed a low protein content of 27.5% and no significant hydrolysis. The use of protease combined with calcium lactate increased the protein content to 55.8% with a degree of hydrolysis of 9.77%, which is attributed to the breakdown of globular proteins and improved solubility. The highest efficiency was achieved using combined enzymatic hydrolysis with preliminary amylase treatment, resulting in a protein content of 70.5%, an amino nitrogen level of 698.66 mg per 100 g, and a degree of hydrolysis of 13.41%. The reduction in dry matter yield from 28.5 to 16.8 g confirms effective removal of non protein components, primarily starch and dietary fiber. It is shown that amylase decreases system viscosity and increases protein availability for protease action, while calcium lactate enhances protein coagulation and phase separation. The results demonstrate the feasibility of combined enzymatic hydrolysis for producing high protein plant ingredients with improved bioavailability recommended for functional and specialized food products.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>растительный белок</kwd><kwd>горох</kwd><kwd>ферментативный гидролиз</kwd><kwd>протеаза</kwd><kwd>амилаза</kwd><kwd>лактат кальция</kwd><kwd>выделение белка</kwd><kwd>гидролизат белка</kwd><kwd>степень гидролиза</kwd></kwd-group><kwd-group xml:lang="en"><kwd>vegetable protein</kwd><kwd>pea</kwd><kwd>enzymatic hydrolysis</kwd><kwd>protease</kwd><kwd>amylase</kwd><kwd>calcium lactate</kwd><kwd>protein isolation</kwd><kwd>protein hydrolysate</kwd><kwd>degree of hydrolysis</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Чугунова О.В., Бикбулатов П.С., Девяткин Д.И. и др. Ресурсный потенциал сортов гороха Свердловской области для получения белковых гидролизатов // Индустрия питания. 2025. Т. 10. № 3. С. 53–61. doi: 10.29141/2500-1922-2025-10-3-6</mixed-citation><mixed-citation xml:lang="en">Chugunova O.V., Bikbulatov P.S., Devyatkin D.I. et al. Resource potential of pea varieties from the Sverdlovsk region for obtaining protein hydrolysates. Food Industry. 2025. vol. 10. no. 3. pp. 53–61. doi: 10.29141/2500-1922-2025-10-3-6 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Иванчихина О.В. Значение белка в профилактике и лечении заболеваний // Медицина. Социология. Философия. Прикладные исследования. 2023. № 2. С. 59–62.</mixed-citation><mixed-citation xml:lang="en">Ivanchikhina O.V. The importance of protein in the prevention and treatment of diseases. Medicine. Sociology. Philosophy. Applied Research. 2023. no. 2. pp. 59–62. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Куликов Д.С., Аксенова Л.М., Самойлова А.М. Функционально-технологические свойства белковых продуктов из зернобобовых культур и их модификация под влиянием различных факторов. Часть 1 // Пищевая промышленность. 2024. № 3. С. 20–25. doi: 10.52653/PPI.2024.3.3.004</mixed-citation><mixed-citation xml:lang="en">Kulikov D.S., Aksenova L.M., Samoilova A.M. Functional and technological properties of protein products from legumes and their modification under the influence of various factors. Part 1. Food Industry. 2024. no. 3. pp. 20–25. doi: 10.52653/PPI.2024.3.3.004 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Позняковский В.М. Вызовы и стратегические мегатренды современной нутрициологии // Индустрия питания. 2024. Т. 9. № 2. С. 5–12. doi: 10.29141/2500-1922-2024-9-2-1</mixed-citation><mixed-citation xml:lang="en">Poznyakovsky V.M. Challenges and strategic megatrends of modern nutriology. Food Industry. 2024. vol. 9. no. 2. pp. 5–12. doi: 10.29141/2500-1922-2024-9-2-1 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Куликов Д.С., Королев А.А. Функционально-технологические свойства белковых продуктов из зернобобовых культур и их модификация под влиянием различных факторов. Часть 2 // Пищевая промышленность. 2024. № 8. С. 35–44. doi: 10.52653/PPI.2024.8.8.007</mixed-citation><mixed-citation xml:lang="en">Kulikov D.S., Korolev A.A. Functional and technological properties of protein products from legumes and their modification under the influence of various factors. Part 2. Food Industry. 2024. no. 8. pp. 35–44. doi: 10.52653/PPI.2024.8.8.007 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Бикбулатов П.С., Чугунова О.В., Заворохина Н.В. Анализ современного рынка растительных белков и технологических решений их получения // Вестник КрасГАУ. 2025. № 5 (218). С. 244–260. doi: 10.36718/1819-4036-2025-5-244-260</mixed-citation><mixed-citation xml:lang="en">Bikbulatov P.S., Chugunova O.V., Zavorokhina N.V. Analysis of the modern plant protein market and technological solutions for their production. Bulletin of KrasGAU. 2025. no. 5. pp. 244–260. doi: 10.36718/1819-4036-2025-5-244-260 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Matoba T., Hata T. Relationship between bitterness of peptides and their chemical structures // Agricultural and Biological Chemistry. 1972. V. 36. P. 1423–1431.</mixed-citation><mixed-citation xml:lang="en">Matoba T., Hata T. Relationship between bitterness of peptides and their chemical structures. Agricultural and Biological Chemistry. 1972. vol. 36. no. 8. pp. 1423–1431. doi: 10.1080/00021369.1972.10860458</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Leksrisompong P.P., Miracle R.E., Drake M.A. Characterization of Flavor of Whey Protein Hydrolysates // Journal of Agricultural and Food Chemistry. 2010. V. 58. № 10. P. 6318–6327.</mixed-citation><mixed-citation xml:lang="en">Leksrisompong P.P., Miracle R.E., Drake M.A. Characterization of flavor of whey protein hydrolysates. Journal of Agricultural and Food Chemistry. 2010. vol. 58. no. 10. pp. 6318–6327. doi: 10.1021/jf9044297</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Chatterjee C., Gleddie S., Xiao C.W. Soybean bioactive peptides and their functional properties // Nutrients. 2018. V. 10. № 9. P. 1211. doi: 10.3390/nu10091211</mixed-citation><mixed-citation xml:lang="en">Chatterjee C., Gleddie S., Xiao C.W. Soybean bioactive peptides and their functional properties. Nutrients. 2018. vol. 10. no. 9. p. 1211. doi: 10.3390/nu10091211</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Куликов Д.С., Королев А.А. Аспекты ферментативной модификации растительных белков // Пищевые системы. 2025. Т. 8. № 1. С. 22–28. doi: 10.21323/2618-9771-2025-8-1-22-28</mixed-citation><mixed-citation xml:lang="en">Kulikov D.S., Korolev A.A. Aspects of enzymatic modification of plant proteins. Food Systems. 2025. vol. 8. no. 1. pp. 22–28. doi: 10.21323/2618-9771-2025-8-1-22-28 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Матишова Д.А., Приходько Д.В., Красноштанова А.А. Получение белковых изолятов на основе муки бобовых // Бутлеровские сообщения. 2025. Т. 81. № 1. С. 114–124. doi: 10.37952/ROI-jbc-01/25-81-1-114</mixed-citation><mixed-citation xml:lang="en">Matishova D.A., Prikhodko D.V., Krasnoshtanova A.A. Production of protein isolates based on legume flour. Butlerov Communications. 2025. vol. 81. no. 1. pp. 114–124. doi: 10.37952/ROI-jbc-01/25-81-1-114 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Витол И.С. Характеристика продуктов ферментативной модификации семян бобовых культур // Пищевая промышленность. 2024. № 10. С. 73–76. doi: 10.52653/PPI.2024.10.10.014</mixed-citation><mixed-citation xml:lang="en">Vitol I.S. Characteristics of products of enzymatic modification of legume seeds. Food Industry. 2024. no. 10. pp. 73–76. doi: 10.52653/PPI.2024.10.10.014 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Шариков А.Ю., Иванов В.В., Амелякина М.В. Влияние перемешивания на эффективность ферментативного гидролиза высококонцентрированных сред экструдированного крахмала кукурузы // Вестник Воронежского государст-венного университета инженерных технологий. 2020. Т. 82. № 3. С. 96–103. doi: 10.20914/2310-1202-2020-3-96-103</mixed-citation><mixed-citation xml:lang="en">Sharikov A.Yu., Ivanov V.V., Amelyakina M.V. The effect of stirring on the efficiency of enzymatic hydrolysis of high-concentration media of extruded corn starch. Bulletin of Voronezh State University of Engineering Technologies. 2020. vol. 82. no. 3. pp. 96–103. doi: 10.20914/2310-1202-2020-3-96-103 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Sareen J., Shi D., Stone A.K. et al. Effect of enzyme hydrolysis on the physicochemical, functional, and nutritional properties of pea and faba bean protein isolates // European Food Research and Technology. 2023. V. 249. № 12. P. 3175–3190. doi: 10.1007/s00217-023-04358-y</mixed-citation><mixed-citation xml:lang="en">Sareen J., Shi D., Stone A.K. et al. Effect of enzyme hydrolysis on the physicochemical, functional, and nutritional properties of pea and faba bean protein isolates. European Food Research and Technology. 2023. vol. 249. no. 12. pp. 3175–3190. doi: 10.1007/s00217-023-04358-y</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Gharibzahedi S.M.T., Smith B., Altintas Z. Bioactive and health-promoting properties of enzymatic hydrolysates of legume proteins: a review // Critical Reviews in Food Science and Nutrition. 2024. V. 64. № 9. P. 2548–2578. doi: 10.1080/10408398.2022.2124399</mixed-citation><mixed-citation xml:lang="en">Gharibzahedi S.M.T., Smith B., Altintas Z. Bioactive and health-promoting properties of enzymatic hydrolysates of legume proteins: a review. Critical Reviews in Food Science and Nutrition. 2024. vol. 64. no. 9. pp. 2548–2578. doi: 10.1080/10408398.2022.2124399</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">García Arteaga V., Leeb E., Liu Y. et al. Enzymatic hydrolysis and fermentation of pea protein isolate and its effects on antigenic proteins, functional properties, and sensory profile // Foods. 2022. V. 11. № 1. P. 118. doi: 10.3390/foods11010118</mixed-citation><mixed-citation xml:lang="en">García Arteaga V., Leeb E., Liu Y. et al. Enzymatic hydrolysis and fermentation of pea protein isolate and its effects on antigenic proteins, functional properties, and sensory profile. Foods. 2022. vol. 11. no. 1. p. 118. doi: 10.3390/foods11010118</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Tulbek M.C., Wang Y.L., Hounjet M. Pea—a sustainable vegetable protein crop // Sustainable Protein Sources / Ed. by S.R. Nadathur, J.P.D. Wanasundara, L. Scanlin. 2nd ed. Cambridge: Academic Press, 2024. P. 143–162. doi: 10.1016/B978-0-323-91652-3.00027-7</mixed-citation><mixed-citation xml:lang="en">Tulbek M.C., Wang Y.L., Hounjet M. Pea—a sustainable vegetable protein crop. Sustainable Protein Sources. 2nd ed. Ed. by S.R. Nadathur, J.P.D. Wanasundara, L. Scanlin. Cambridge: Academic Press, 2024. pp. 143–162. doi: 10.1016/B978-0-323-91652-3.00027-7</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Shanthakumar P., Klepacka J., Bains A. et al. The current situation of pea protein and its application in the food industry // Molecules. 2022. V. 27. № 16. P. 5354. doi: 10.3390/molecules27165354</mixed-citation><mixed-citation xml:lang="en">Shanthakumar P., Klepacka J., Bains A. et al. The current situation of pea protein and its application in the food industry. Molecules. 2022. vol. 27. no. 16. p. 5354. doi: 10.3390/molecules27165354</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">David Troncoso F., Alberto Sánchez D., Luján Ferreira M. Production of plant proteases and new biotechnological applications: an updated review // ChemistryOpen. 2022. V. 11. № 3. P. e202200017. doi: 10.1002/open.202200017</mixed-citation><mixed-citation xml:lang="en">David Troncoso F., Alberto Sánchez D., Luján Ferreira M. Production of plant proteases and new biotechnological applications: an updated review. ChemistryOpen. 2022. vol. 11. no. 3. p. e202200017. doi: 10.1002/open.202200017</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Tiruneh A., Ptaszek P., Żmudziński D. et al. Peas (Pisum sativum subsp. arvense Asch) and Beans (Vicia faba var. minor) as Source of Quality Plant Proteins // Molecules. 2025. V. 30. № 9. P. 2009. doi: 10.3390/molecules30092009</mixed-citation><mixed-citation xml:lang="en">Tiruneh A., Ptaszek P., Żmudziński D. et al. Peas (Pisum sativum subsp. arvense Asch) and Beans (Vicia faba var. minor) as Source of Quality Plant Proteins. Molecules. 2025. vol. 30. no. 9. p. 2009. doi: 10.3390/molecules30092009</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru"></mixed-citation><mixed-citation xml:lang="en"></mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
