Microbiological aspects of the effect of Fe NPs on Wistar rats
https://doi.org/10.20914/2310-1202-2019-3-168-173
Abstract
An important route of metal intake from NP preparations is the pathway through absorption by bacterial cells of the gastrointestinal tract. This changes the composition of the microflora. Thus, giving animals NP Cu is accompanied by an increase in the total number of bacteria and lactobacilli, and a decrease in the population of E. coli and Clostridium spp. When it enters the intestine, part of the NP is able to form a pool on the inner wall of the intestine, in connection with which it can act with microorganisms inhabiting the gastrointestinal tract. The intestinal microbiota is known to play a vital role in the nutritional and immunological functions of host animals. However, in the literature there is not enough data on the influence of NP on the intestinal microbiocenosis of various representatives of the animal world and humans. The aim of the study is to study the effect of Fe nanoparticles introduced by per os on representatives of the main physiological groups of microorganisms. 30 male Wistar rats aged 4 months, identical in weight (from 180 g), were selected on the basis of analogues. up to 250 g.), physiological state, were in the previous period of experience in a balanced diet on the recommendations. Preparations of NP Fe for the introduction of per os were prepared in isotonic saline solution, treated for 30 minutes on an ultrasonic dispersant. Nanoparticles in the required amount were mixed with rice. The frequency of giving NP Fe to animals in order to correct microbiocenosis with deficient diets is 1 time in 7 days. In this case, the introduction of 10 mg of iron per kg of animal weight showed the best results.
About the Authors
A. M. Korotkova
Federal Scientific Center of Biological systems and agro-technologies of the Russian Academy of Sciences
Russian Federation
Cand. Sci. (Biol.), experimental-biological clinic (vivarium), Orenburg, 9 January, 29
Russian Federation
Cand. Sci. (Biol.), experimental-biological clinic (vivarium), Orenburg, 9 January, 29
O. V. Kvan
Federal Scientific Center of Biological systems and agro-technologies of the Russian Academy of Sciences
Cand. Sci. (Biol.), senior researcher, Orenburg, 9 Yanvarya 29
Cand. Sci. (Biol.), senior researcher, Orenburg, 9 Yanvarya 29
I. A. Vershinina
Orenburg State University
graduate student, ,, Orenburg, Pobedy sq., 13
graduate student, ,, Orenburg, Pobedy sq., 13
S. V. Lebedev
Orenburg state university
Dr. Sci. (Biol.), biotechnology of animal raw materials and aquaculture department, Orenburg, Pobedy sq., 13
Dr. Sci. (Biol.), biotechnology of animal raw materials and aquaculture department, Orenburg, Pobedy sq., 13
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Review
For citations:
Korotkova A.M., Kvan O.V., Vershinina I.A., Lebedev S.V. Microbiological aspects of the effect of Fe NPs on Wistar rats. Proceedings of the Voronezh State University of Engineering Technologies. 2019;81(3):168-173. (In Russ.) https://doi.org/10.20914/2310-1202-2019-3-168-173
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