In vitro release studies of rilpivirine from in situ forming polymeric implants in buffer solution and in a gel phantom of muscle tissue
https://doi.org/10.20914/2310-1202-2023-4-70-75
Abstract
Development of in situ forming implants (ISFI) based on PLGA polymers is one of the most promising approaches to long-acting injectables. Evaluation of the drug release rate from such depot formulations requires methods that most closely simulate in vivo conditions. Gel phantoms mimic the elastic properties of muscle tissue and appear to be a promising replacement for conventional methods using physiologically relevant buffer solutions. Accordingly, the aim of the study was to select the optimal composition for the gel phantom formation and evaluate the effect of the phantom matrix on the release rate of rilpivirin used as a model drug from the PLGA ISFI. According to the results of the study, a 1% agarose gel was the best suited for a tissue phantom preparation and implant formation. It was also shown that the release profile of rilpivirin from the ISFI matrix depended on how the implant was formed (in a gel or freely in buffer). In the case of a phantom, the structure of the implant was less porous and retained its shape for 28 days of incubation at 37 °C. During this period, the ISFI formed in an agarose gel released considerably less rilpivirin compared to the ISFI formed without gel (11% vs 80%).
About the Authors
Y. V. Ulianova
D. Mendeleev University of Chemical Technology of Russia
Russian Federation
Russian Federation
Cand. Sci. (Chem.), assistant, chemistry and technology of biomedical preparations department, Miusskaya square 9, Moscow, 125047, Russia
Y. V. Ermolenko
D. Mendeleev University of Chemical Technology of Russia
Cand. Sci. (Chem.), associate professor, chemistry and technology of biomedical preparations department, Miusskaya square 9, Moscow, 125047, Russia
L. V. Vanchugova
A.V. Topchiev Institute of Petrochemical Synthesis Russian Academy of Sciences
Cand. Sci. (Chem.), leading researcher, laboratory of chemistry of polyelectrolytes and biomedical polymers, Leninsky prospect 29, Moscow, 119991, Russia
A. V. Mityukov
A.V. Topchiev Institute of Petrochemical Synthesis Russian Academy of Sciences
Cand. Sci. (Chem.), junior researcher, laboratory of polymer rheology, Leninsky prospect 29, Moscow, 119991, Russia
S. E. Gelperina
D. Mendeleev University of Chemical Technology of Russia
Dr. Sci. (Chem.), professor, chemistry and technology of biomedical preparations department, Miusskaya square 9, Moscow, 125047, Russia
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Review
For citations:
Ulianova Y.V., Ermolenko Y.V., Vanchugova L.V., Mityukov A.V., Gelperina S.E. In vitro release studies of rilpivirine from in situ forming polymeric implants in buffer solution and in a gel phantom of muscle tissue. Proceedings of the Voronezh State University of Engineering Technologies. 2023;85(4):70-75. (In Russ.) https://doi.org/10.20914/2310-1202-2023-4-70-75
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