Bioelectrocatalytic oxygen reduction by bacterial laccase on electrodes modified with multi-walled carbon nanotubes
https://doi.org/10.20914/2310-1202-2024-2-126-131
Abstract
In this work, the electrochemical behaviour of bioelectrodes based on bacterial laccase Streptomyces carpinensis VKM Ac-1300 obtained using different immobilization methods was investigated. The bioelectrodes were formed by fixing the enzyme on the electrode surface by simple adsorption, by adsorption on the modified electrode by multi-walled carbon nanotubes and by covalent bounding of the enzyme by carboxyl groups with functionalized multi-walled carbon nanotubes. The orientation of immobilized laccase enzymes and their ability to direct and mediated electron transfer were assessed by direct amperometry at constant potential. It was found that, depending on the method of immobilization, from 5 to 10% of immobilized enzyme has the correct orientation and, consequently, the ability to direct electron transfer. At the same time, covalent bounding of protein on the surface of graphite electrodes modified with nanotubes leads to a more active direct electron transfer, an increase in the rate of oxygen reduction and long-term electrode stability. Thus, for bacterial laccase Streptomyces carpinensis VKM Ac-1300 the possibility of direct electron transfer at their immobilization by covalent bounding with carboxyl groups of multi-walled carbon nanotubes was shown. The developed bioelectrodes can be used as cathodes in biofuel cells.
About the Authors
S. V. Alferov
Research canter "BioChemTech" Tula State University
Russian Federation
Russian Federation
Cand. Sci. (Engin.), head of the laboratory, biotechnology, laboratory of ecological and medical biotechnology department, Friedrich Engels Street 157, 300012 Tula, Russia
V. V. Fedina
Research center "BioChemTech" Tula State University
assistant, junior researcher, biotechnology, laboratory of ecological and medical biotechnology department, Friedrich Engels Street 157, 300012 Tula, Russia
K. A. Egorov
research center "BioChemTech" Tula State University
junior researcher, laboratory of ecological and medical biotechnology, Friedrich Engels Street 157, 300012 Tula, Russia
L. I. Trubitsina
Institute of Biochemistry and Physiology of Microorganisms RAS – A Separate Subdivision of Pushchino Scientific Center for Biological Research RAS
Cand. Sci. (Biol.), researcher, laboratory of microbe enzymology, Prospekt Nauki, 5, Pushchino, Moscow region, 142290, Russia
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Review
For citations:
Alferov S.V., Fedina V.V., Egorov K.A., Trubitsina L.I. Bioelectrocatalytic oxygen reduction by bacterial laccase on electrodes modified with multi-walled carbon nanotubes. Proceedings of the Voronezh State University of Engineering Technologies. 2024;86(2):126-131. (In Russ.) https://doi.org/10.20914/2310-1202-2024-2-126-131
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