Estimation of biotic potential in potatoes and apples
https://doi.org/10.20914/2310-1202-2025-1-168-174
Abstract
The study focuses on the determination of biotic potential in potatoes and apples using Tetrahymena pyriformis infusoria. Rapid control methods are becoming increasingly important due to the need to rapidly assess quality and safety parameters. Tetrahymena pyriformis is a convenient test system for biotesting due to its high sensitivity to changes in the environment. The method of biotesting on infusoria allows rapid and inexpensive evaluation of the biological activity of products, their safety and general toxicity. Samples of potatoes and apples, chopped to homogeneous state and introduced into nutrient medium were used in the study. After incubation for 48 h, the number of microorganisms was counted to calculate the biotic potential value. The results showed that the highest growth of microorganisms was observed in all samples with 1 g product content. The least growth was recorded at 4 g content for apple samples and 0.5 g for potato samples. This is linked to the chemical composition of the products: potatoes are predominantly starchy and apples consist of simple sugars, which affects the growth of the number of infusoria. The findings of the study emphasise the strong dependence of biotic potential on the chemical composition and amount of added products. Further research is recommended to form mathematical correlations with the possibility of applying these correlations to develop formulations of products with the best digestibility. The use of infusoria for biotesting allows effective control of product quality and identification of potential health risks for consumers.
About the Authors
A. E. Dzhabakova
BIOTECH University
Russian Federation
Russian Federation
senior lecturer, food safety department, 11 Volokolamskoe Shosse, Moscow, 125080, Russia
T. V. Tulyakova
BIOTECH University
Dr. Sci. (Tech.), professor, food safety department, 11 Volokolamskoe Shosse, Moscow, 125080, Russia
N. V. Fomenko
BIOTECH University
Cand. Sci. (Tech.), associate professor, food safety department, 11 Volokolamskoe Shosse, Moscow, 125080, Russia
I. B. Leonova
Plekhanov Russian University of Economics
Cand. Sci. (Tech.), associate professor, Department of Commodity Expertise and Customs Affairs, 36 Stremiany pereylok, Moscow 115054, Russia
References
1. Karpenko Yu. V., Krashchenko V.V. Biotesting of fish culinary products using Tetrahymena pyriformis. Bulletin of Astrakhan State Technical University. Series: Fishery. 2019. no. 3. pp. 132–140. (in Russian).
2. Sorokoletov O.N., Gaptar S.L., Bgatov A.V. Application of Biotesting for Assessment of Biological Value of Food Products. Innovations and Food Safety. 2022. vol. 38. no. 4. pp. 31–36. DOI: 10.31677/2311–0651–2022–38–4–31–36. (in Russian).
3. Application of Paramecium caudatum for the assessment of energy costs for food raw materials and products digestibility – February 2021 IOP Conference Series Earth and Environmental Science 640(6):062006 – DOI:10.1088/1755–1315/640/6/062006
4. Bondaruk A.M., Tsygankov V.G., Zhurikhina L.N., Fedorovich S.V., Gulin V.V. Assessment of biological value and harmlessness of food products in order to develop rations for tourism and recreational activities. Proceedings of BSTU. Series 1: Forestry, nature management and processing of renewable resources. 2016. no. 1 (183). pp. 225–230. (in Russian).
5. Plotnikova I.V., Zharkova I.M., Grebenschikov A.V., Rogov K.O., Plotnikov V.E. Study of biological activity of aqueous suspensions of legume cultures as an alternative to egg white in confectionery products of foamy structure. Voprosy Children's Dietetics. 2021. vol. 19. no. 2. pp. 99–106. DOI: 10.20953/1727–5784–2021–2–99–106. (in Russian).
6. Gerhardt, A., Ud-Daula, A., Schramm, K. – W. Tetrahymena spp. (Protista, Ciliophora) as Test Species in Rapid Multilevel Ecotoxicity Tests. Acta Protozoologica. 2010. V. 49, № 4. P. 271–280.
7. Luan, F., Wang, T., Tang, L., Zhang, S., Cordeiro, M.N.D.S. Estimation of the Toxicity of Different Substituted Aromatic Compounds to the Aquatic Ciliate Tetrahymena pyriformis by QSAR Approach. Molecules. 2018. V. 23, № 5. P. 1002–1017. DOI: 10.3390/molecules23051002
8. Korovina N.S. Development of probiotic sour-milk product of antioxidant orientation. Bulletin of South Ural State University. Series: Food and biotechnology. 2024. vol. 12. no. 1. pp. 34–42. DOI: 10.14529/food240104. (in Russian).
9. Leonova I.B., Babanina V.I. Study of the possibility of using biotesting for comparative assessment of juice quality. Economics: yesterday, today, tomorrow. 2019. 9 (1А), pp. 419–427.
10. Perfilova O.V., Bryksina K.V., Grebenschikov A.V. Evaluation of biotic potential and biological value of rye-wheat bread with fruit and vegetable pastes. Technologies of food and processing industry AIC – products of healthy nutrition. 2022. no. 4. pp. 39–44. (in Russian).
11. State Standard 24230–80. Vegetable feeds. Method for determination of in vitro digestibility. Moscow, IPK Izdatel standards Publ., 2003. 4 p. (in Russian).
12. State Standard 31674–2012. Fodders, mixed fodders, mixed fodder raw materials. Methods of determination of general toxicity. Moscow, Standartinform Publ., 2014. 39 p. (in Russian).
13. Quality СHECK – application for food production: official site. – Moscow, 2023. – URL: https://quality-check.ru/ (accessed 12.02.25).
14. Chemical composition of Russian food products: Reference book. Moscow: DeLee Print. 2002. 236 p.
Review
For citations:
Dzhabakova A.E., Tulyakova T.V., Fomenko N.V., Leonova I.B. Estimation of biotic potential in potatoes and apples. Proceedings of the Voronezh State University of Engineering Technologies. 2025;87(1):168-174. (In Russ.) https://doi.org/10.20914/2310-1202-2025-1-168-174
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