Development of a method for producing soil conditioner based on acrylic hydrogel modified with biofungicide ‘Trichodermin’
https://doi.org/10.20914/2310-1202-2026-3-344-353
Abstract
The method of encapsulating biopesticides in polymer matrices is actively used to increase crop yields. However, existing research has focused primarily on the use of natural polymer matrices, which are significantly inferior to synthetic polymers in a number of performance characteristics. Copolymers of acrylic acid and acrylamide have proven themselves as soil conditioners due to their high sorption characteristics, ease of chemical modification, and the reproducibility of their properties. In the presented work, three methods of acrylic hydrogel synthesis were investigated, differing in the stages of the process and methods of introducing the biofungicide "Trichodermin". Using IR spectroscopy, optical microscopy and microbiological analysis, it was shown that all the methods used to obtain the soil conditioner allow the functional activity of T. harzianum to be preserved after its encapsulation in acrylic hydrogel. However, the study of the sorption characteristics and parameters of polymer mesh showed that the optimal method is the introduction of biofungicide at the synthesis stage, which allows for high sorption characteristics: the values of the equilibrium degree of swelling in water and in physiological solution were, respectively, Q∞ = 410 g/g and Q∞ = 50 g/g. Such values are achieved by forming a low-defect polymer mesh with a low content of sol fraction (S=0.04) and a high proportion of active chains (Vc ~ 0.70). The resulting material is promising for use as a soil conditioner with prolonged biofungicide release.
About the Authors
I. V. krylachRussian Federation
M. V. Uspenskaya
Russian Federation
R. O. Olekhnovich
Russian Federation
N. V. Chernikova
Russian Federation
References
1. Balla A., Silini A., Cherif-Silini H., Chenari Bouket A. et al. Recent advances in encapsulation techniques of plant growth-promoting microorganisms and their prospects in the sustainable agriculture. Applied Sciences. 2022. vol. 12. no. 18. pp. 9020. doi:10.3390/app12189020.
2. Singh G., Paithankar I. Encapsulation of biofertilizers, biopesticides and biocontrol agents. In Sustainable Agriculture Reviews 60: Microbial Processes in Agriculture Cham: Springer Nature Switzerland. 2023. pp. 121-150. doi: 10.1007/978-3-031-24181-9_6.
3. De Souza Coelho C. C., da Silva T. B. M., de Lima A. C. P., dos Santos Mota M. F. et al. The role of encapsulation in promoting fungi and bacteria as biopesticides: Insights from a Bibliometric Review. Biocatalysis and Agricultural Biotechnology. 2025. vol. 70. pp. 103802. doi: 10.1016/j.bcab.2025.103802.
4. Rathore S., Desai P. M., Liew C. V., Chan L. W. et al. Microencapsulation of microbial cells. Journal of food engineering. 2013. vol. 116. no. 2. pp. 369-381. doi: 10.1016/j.jfoodeng.2012.12.022.
5. Tyśkiewicz R., Nowak A., Ozimek E., Jaroszuk-Ściseł J. Trichoderma: The current status of its application in agriculture for the biocontrol of fungal phytopathogens and stimulation of plant growth. International journal of molecular sciences. 2022. vol. 23. no. 4. pp. 2329. doi: 10.3390/ijms23042329.
6. Guzmán-Guzmán P., Kumar A., de Los Santos-Villalobos S., Parra-Cota F. I. et al. Trichoderma species: Our best fungal allies in the biocontrol of plant diseases—A review. Plants. 2023. vol. 12. no. 3. pp. 432. doi: 10.3390/plants12030432.
7. Ding H., Li X., Wang S., Yang Y. et al. Trichoderma harzianum for the control of agricultural pests: Potential, progress, applications and future prospects. Revista Argentina de Microbiología. 2025. vol. 58. no. 1. pp. 101-118. doi:10.1016/j.ram.2025.09.004.
8. Phuengsanthia K., Bussaman P., Namsena P., Sa-uth, C. Encapsulation of Trichoderma harzianum with sodium alginate and evaluation of efficacy against fungal plant pathogen. Food Agricultural Sciences and Technology. 2024. vol. 10. no.3. pp. 55-67. doi: 10.14456/fast.2024.20.
9. Malavathu K., Godbole V., Singh A., Chandrika K. P. et al. Porous biopolymer matrices: advanced seed delivery platforms for beneficial microbes to combat Soilborne diseases. ACS omega. 2025. vol. 10. no. 20. pp. 20723-20731. doi:10.1021/acsomega.5c01710.
10. Muñoz-Celaya A. L., Ortiz-García M., Vernon-Carter E. J., Jauregui-Rincón J. et al. Spray-drying microencapsulation of Trichoderma harzianum conidias in carbohydrate polymers matrices. Carbohydrate polymers. 2012. vol. 88. no.4. pp. 1141-1148. doi:10.1016/j.carbpol.2011.12.030.
11. Martínez-Vela L. D. J., Montero-Cortés M. I., Qui-Zapata J. A., Farias-Cervantes V. S. et al. Gelatin–Chitosan–PVA Hydrogels Incorporating Trichoderma and Their Application in the Control of Phytopathogens. Gels. 2026. vol. 12. no. 2. pp. 144. doi: 10.3390/gels12020144.
12. Bae Y. S., Knudsen G. R. Effect of sclerotial distribution pattern of Sclerotinia sclerotiorum on biocontrol efficacy of Trichoderma harzianum. Applied Soil Ecology. 2007. vol. 35. no. 1. pp. 21-24. doi:10.1016/j.apsoil.2006.05.014.
13. Rahimi Mamaghani K., Alikarami M., Saremi H. Polymeric Hydrogels in Agriculture: Environmental Performance, Sustainability Challenges, and Future Perspectives. ACS Agricultural Science & Technology. 2025. vol. 5. no.12. pp. 2341-2360. doi:10.1021/acsagscitech.5c00808.
14. Peppas N. A., Franson, N. M. The swelling interface number as a criterion for prediction of diffusional solute release mechanisms in swellable polymers. Journal of polymer science: polymer physics edition. 1983. vol. 21. no. 6. pp. 983-997. doi: 10.1002/pol.1983.180210614.
15. Ganji F., Vasheghani-Farahani S., Vasheghani-Farahani E. Theoretical description of hydrogel swelling: a review. Iranian Polymer Journal. 2010. vol. 19. no.5. pp. 375-398.
16. Shoaib A., Aslam N., Aslam N. Trichoderma harzianum: adsorption, desorption, isotherm and FTIR studies. The Journal of Animal and Plant Sciences.2013. vol. 23. no. 5. pp. 1460-1465.
17. Chaverri P., Branco-Rocha F., Jaklitsch W., Gazis R. et al. Systematics of the Trichoderma harzianum species complex and the re-identification of commercial biocontrol strains. Mycologia. 2015. vol. 107. no.3. pp. 558-590. doi: 10.3852/14-147.
18. Wang J., Wu W., Lin Z. Kinetics and thermodynamics of the water sorption of 2‐hydroxyethyl methacrylate/styrene copolymer hydrogels. Journal of applied polymer science, 2008. vol.109. no.5. pp. 3018-3023. doi: 10.1002/app.28403.
19. Flory P. J. Principles of polymer chemistry. Cornell university press.1953. 672 p.
Review
For citations:
krylach I.V., Uspenskaya M.V., Olekhnovich R.O., Chernikova N.V. Development of a method for producing soil conditioner based on acrylic hydrogel modified with biofungicide ‘Trichodermin’. Proceedings of the Voronezh State University of Engineering Technologies. 2026;88(3):343-352. (In Russ.) https://doi.org/10.20914/2310-1202-2026-3-344-353
JATS XML



























