Global population growth and an increase in anthropogenic pressure on natural resources necessitate the transition to alternative types of food supply. In this aspect, vegetable raw materials could cover the human need for a proportionally increasing dietary protein deficiency. The article presents a method for obtaining protein isolate from partially skimmed rapeseed meal based on extraction with a solution of sodium chloride under conditions of low-frequency mechanical vibrations. The aim of the study is to analyze the effectiveness of low-frequency mechanical vibrations, to identify optimal values of their frequency and amplitude, ensuring effective protein extraction from plant raw materials while preserving its native structure. An analysis of energy efficiency has been carried out, as well as obtaining dynamic characteristics of the mass transfer process. The method involves the use of a vibrating perforated nozzle that performs reciprocating movements with a frequency in the range (10-20 Hz) and an amplitude (3-10 mm), which allows a turbulent hydrodynamic regime to be formed in the processed volume, significantly accelerating the mass transfer process. At the end of the extraction stage, the protein was deposited at an isoelectric point using a solution of succinic acid. Experimental data obtained in laboratory conditions show the possibility of achieving the degree of extraction of vegetable protein up to 90.25% relative to the initial content in the processed raw materials while reducing the extraction time to 15 minutes. The method is characterized by environmental friendliness and the absence of toxic reagents. The results of the study, experimentally repeated three times, showed that the optimal parameters for extracting protein components are a frequency of 15 Hz and an amplitude of 5 mm. An assessment of biochemical parameters was also carried out, the results of which confirm the prospects of using vibration technology in the food and biotechnological industries for the production of functional protein products.

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