Modeling of hydrodynamics in hollow fiber membrane bioreactor for mammalian cells cultivation
https://doi.org/10.20914/2310-1202-2016-3-82-87
Abstract
The mathematical modelling in CFD-packages are powerfull instrument for design and calculation of any engineering tasks. CFD-package contains the set of programs that allow to model the different objects behavior based on the mathematical lows. ANSYS Fluent are widely used for modelling of biotechnological and chemical-technological processes. This package is convenient to describe their hydrodynamics. As cell cultivation is one of the actual scientific direction in modern biotechnology ANSYS Fluent was used to create the model of hollow fiber membrane bioreactor. The fibers are hollow cylindrical membrane to be used for cell cultivation. The criterion of process effectiveness for cell growth is full filling of the membrane surface by cells in the bioreactor. While the cell growth the fiber permeability is decreased which effects to feed flow through membrane pores. The specific feature of this process is to ensure such feed flow to deliver the optimal nutrition for the cells on the external membrane surface. The velocity distribution inside the fiber and in all bioreactor as a whole has been calculated based on mass an impulse conservation equations taking into account the mathematical model assumptions. The hydrodynamics analysis in hollow fiber membrane bioreactor is described by the three-dimensional model created in ANSYS Fluent. The specific features of one membrane model are considered and for whole bioreactor too.
About the Authors
N. V. Menshutina
D.I. Mendeleev University of Chemical Technology of Russia, Miusskaya Sq., 9, Moscow, 125047, Russia
Russian Federation
doctor of technical sciences, full professor, department of cybernetic of chemical engineering processes
Russian Federation
doctor of technical sciences, full professor, department of cybernetic of chemical engineering processes
R. R. Safarov
Ltd. “Cell systems”, Fersmana Str., 5A, Moscow, Russia
Russian Federation
Russian Federation
Applicant, Department of Cybernetic of Chemical Technology Processes;
Head of Administrative Procedures Department
E. S. Vorobeva
D.I. Mendeleev University of Chemical Technology of Russia, Miusskaya Sq., 9, Moscow, 125047, Russia
Russian Federation
graduate student, department of cybernetic of chemical technology processes
Russian Federation
graduate student, department of cybernetic of chemical technology processes
E. V. Guseva
D.I. Mendeleev University of Chemical Technology of Russia, Miusskaya Sq., 9, Moscow, 125047, Russia
Russian Federation
doctor of technical sciences, associate professor, department of cybernetic of chemical engineering processes
Russian Federation
doctor of technical sciences, associate professor, department of cybernetic of chemical engineering processes
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Review
For citations:
Menshutina N.V., Safarov R.R., Vorobeva E.S., Guseva E.V. Modeling of hydrodynamics in hollow fiber membrane bioreactor for mammalian cells cultivation. Proceedings of the Voronezh State University of Engineering Technologies. 2016;(3):82-87. (In Russ.) https://doi.org/10.20914/2310-1202-2016-3-82-87
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