Study of the influence of biostimulants on the activity of bacterial and fungal hydrolases

Abstract

One of the promising areas of biotechnology is the bioconversion of plant raw materials, an example of which is solid-phase cultivation: the cultivation of microorganisms and higher fungi on plant raw materials in order to obtain biologically active substances, for example, cellulases. At the moment, there are data on studies on methods of enhancing the cellulase activity of microorganisms by modifying nutrient media: the addition of vitamins, minerals, bioregulators. In this work, strains of microorganisms of the genera Bacillus, Alternaria, Trichoderma were used. These microorganisms are capable of biodegradation of cellulose and were obtained from the All-Russian Collection of Microorganisms. These cultures of microorganisms are planned to be used as destructors of cellulose in sunflower meal. Appinee (2,4-epibrassinolide) and heteroauxin (?-indolylacetic acid) bioregulators were used as amplifiers of cellulolytic activity. To determine the cellulolytic activity, the Hutchinson medium method with the addition of 10 g / l of carbomethylcellulose was used, and the ability of the Congo-red dye to form a complex with cellulose. The diameter of the bleaching zone around the wells served as an estimate of the presence of cellulolytic activity. It was found that the appine bioregulator in the dilution 10-1 showed the greatest increase in cellulolytic activity. Further solid-phase fermentation was carried out on a medium based on sunflower meal. After analyzing the data obtained during the experiments, it can be concluded that the stimulant appine and heteroauxin have a positive effect on the cellulolytic activity of Alternaria alternate, Trichoderma reesei, Bacillus subtilis, with the maximum effect being on appine