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Selection of optimal conditions for inducing the biosynthesis of recombinant lipase LipA

https://doi.org/10.20914/2310-1202-2013-2-191-194

Abstract

The influence of the conditions of biosynthesis (pH, t °, the cultivation time, the concentration of IPTG-inducer, time of IPTG application and time of biosynthesis) on obtaining the maximum amount of the enzyme LipA.

About the Authors

V. A. Annenkov
Воронеж. гос. ун-т инж. технол.
Russian Federation
аспирант, кафедра микробиологии и биохимии


E. A. Pershina
Воронеж. гос. ун-т инж. технол.
Russian Federation
студент, кафедра микробиологии и биохимии


D. A. Cherenkov
Воронеж. гос. ун-т инж. технол.
Russian Federation
доцент, кафедра микробиологии и биохимии


O. S. Korneeva
Воронеж. гос. ун-т инж. технол.
Russian Federation
профессор, кафедра микробиологии и биохимии


References

1. 1 Cherenkov, D. A. Production of thermostable lipases using computer modeling and genetic engineering [Text] / D. A. Cherenkov, V. A. Annenkov, E. V. Pershyna et al // Actual biotechnology. - 2013. - № 3. - P. 34-35.

2. 2 Polygalina, G. V. Determination of enzyme activity [Text] / G. V. Polygalina, V. S. Cherednychenko, L. V. Rimareva. – M.: DeLee print, 2003 - 375 p.

3. 3 Ahmad, S. Thermostable Bacillus subtilis lipases: in vitro evolution and structural insight [Text] / S. Ahmad, M. Z. Kamal, R. Sankaranarayanan et al // J. Mol. Biol. – 2008. -№ 381 (2). - P. 324-340.

4. 4 Don, S. M. Optimal conditions for the growth of E. coli [Text] / S. M. Don // Biology EEI.- 2008. - № 4. - P. 3-18.

5. 5 Shariff, F. M. A newly isolated thermostable lipase from Bacillus sp [Text] / F. M. Shariff, R. N. Rahman, M. Basri et al // J. Mol. Sci. – 2011. - № 12(5). - P. 2917 – 34.


Review

For citations:


Annenkov V.A., Pershina E.A., Cherenkov D.A., Korneeva O.S. Selection of optimal conditions for inducing the biosynthesis of recombinant lipase LipA. Proceedings of the Voronezh State University of Engineering Technologies. 2013;(2):191-194. (In Russ.) https://doi.org/10.20914/2310-1202-2013-2-191-194

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ISSN 2226-910X (Print)
ISSN 2310-1202 (Online)