Establishment of probiotic associations for foods and feeds
https://doi.org/10.20914/2310-1202-2024-1-103-107
Abstract
The aim of the study was to design associations of probiotic bacteria using a set of modern methods of microbiological research for the purpose of use in functional food products and feed additives. Strains of lactic acid and propionic acid bacteria were selected according to biological compatibility and absence of competitive interactions. Cultivation and quantification of microorganisms were carried out on MRS medium. Based on the results, 2 consortia of bacteria were constructed containing Lactococcus lactis subsp. lactis 24/48, Lactobacillus рlantarum 578/25, Lactobacillus helveticus 842 (D)-2, Propionibacterium freudenreichii subsp. shermanii As-103/12, Propionibacterium shermanii K-16. The study of biological compatibility of lactic acid bacteria was carried out by drop method. New experimental data on the growth rates of associations and the amount of accumulated biomass at 20 hours of growth were obtained, which is a scientific novelty of this study. It is shown that the character of interstrain interactions is important in the selection of strains for probiotic associations. It was established that the studied strains of lactic acid and propionic acid bacteria in the association should be present in the ratio of 2:1, fermentation with periodic stirring in anaerobic conditions at 37°C and pH 5.9-6.0. Sequential introduction of bacterial cultures is advisable after 3-4 hours of pre-incubation of lactic acid bacteria. Experimental data contain established new regularities. It is noted that for therapeutic and prophylactic effect the concentration of probiotics in dry preparations should be not less than 10 to the extent of 10-12 CFU/g of preparation, so the accumulation of bacterial biomass in the technological process should take into account 10-20% death of the population in the process of drying or concentrating.
About the Author
G. S. Volkova
All-Russian Research Institute of Food Biotechnology
Russian Federation
Russian Federation
Dr. Sci. (Engin.), head of laboratory of biotechnology of organic acids, food and feed additives, All-Russian Research Institute of Food Biotechnology, 111033, Russia
References
1. Khamagaeva I.S., Boyarineva I.V., Potapchuk N.Y. Research of probiotic properties of combined starter. Technics and technology of food production. 2013. no. 1 (28). pp. 54–58. (in Russian).
2. Vorobyeva L.I., Khasaeva F.M., Vasilyuk N.V., Trenkuil E. Characterisation of propionic acid bacteria using matrix-activated laser desorption/ionization (MALDI) mass spectrometry. Microbiology. 2011. vol. 80. no. 5. pp. 651–658. (in Russian).
3. Oleskin A.V., Shenderov B.A. Probiotics and Psychobiotics: the Role of Microbial Neurochemicals. Probiotics & Antimicro. 2019. no. 11. pp. 1071–1085. doi: 10.1007/s12602–019–09583–0
4. Shurkhno R.A. Microbiological preparation based on homofermentative strains of Lactobacillus plantarum isolated from natural sources for biopreservation of plant resources (review of research conducted in the period from 2000 to 2015). Scientific Notes of Kazan University. Series: Natural Sciences. 2016. vol. 158. no. 1. pp. 5–22. (in Russian).
5. Begunova A.V., Rozhkova I.V., Zvereva E.A., Glazunova O.A. et al. Lactic and propionic acid bacteria: the formation of a community for the production of functional products with bifidogenic and hypotensitive properties. Applied Biochemistry and Microbiology. 2019. vol. 55. no. 6. pp. 660–669. doi: 10.1134/S0003683819060048
6. Sultimova T.D., Stoyanova L.G. Study of antibacterial properties of a consortium of microorganisms. Izvestia of higher educational institutions. Volga region. Natural Sciences. 2018. no. 2(22). pp. 42–51. doi: 10.21685/2307–9150–2018–2–4 (in Russian).
7. Sheveleva S.A. Kuvaeva I.B., Efimochkina N.R., Minaeva L.P. Microbiological safety of food: development of regulatory and methodological basis. Nutrition Issues. 2020. vol. 89. no. 4. pp. 125–145. doi: 10.24411/0042–8833–2020–10048 (in Russian).
8. Zimina M.I., Gazieva A.F., Pozo-Dengra J., Noskova S.Y. et al. Determination of the intensity of bacteriocin production by strains of lactic acid bacteria and their effectiveness. Foods and Raw Materials. 2017. vol. 5. no. 1. pp. 108–117. doi: 10.21179/2308–4057–2017–1–108–117
9. Orlova T.N., Ott E.F., Funk I.A., Dorofeev R.V. Regional strains of lactic acid bacteria in bacterial compositions. Dairy Industry. 2020. no. 5. pp. 42–43. doi: 10.31515/1019–8946–2020–05–42–43 (in Russian).
10. Volkova G.S., Serba E.M. Creation of a multistrain bacterial consortium for the technology of probiotic preparations for feed purposes. Technics and technology of food production. 2021. vol. 51. no. 2. pp. 260–269. doi: 10.21603/2074–9414–2021–2–260–269. (in Russian).
11. Glushanova N.A., Blinov A.I., Bakhayev V.V. On the antagonism of probiotic lactobacilli. Epidemiology and Infectious Diseases. 2004. no. 6. pp. 37–39. (in Russian).
12. Hedayati R., Hosseini M., Najafpour G.D. Optimisation of semi-anaerobic vitamin B12 (cyanocobalamin) production from rice bran oil using Propionibacterium freudenreichii PTCC1674. Biocatalysis and Agricultural Biotechnology. 2020. vol. 23. doi: 10.1016/j. bcab.2019.101444
13. Martens J.H., Barg H., Warren M. et al. Microbial production of vitamin B12. Applied Microbiology and Biotechnology. 2002. vol. 58. no. 3. pp. 275–285. doi: 10.1007/s00253–001–0902–7
14. Markowiak P., Śliżewska K. The role of probiotics, prebiotics and synbiotics in animal nutrition. Gut pathogens. 2018. vol. 10. pp. 1-20.
15. Binda S., Hill C., Johansen E., Obis D. et al. Criteria to qualify microorganisms as “probiotic” in foods and dietary supplements. Frontiers in microbiology. 2020. vol. 11. pp. 1662. doi: 10.3389/fmicb.2020.01662
16. Salminen S., Collado M.C., Endo A., Hill C. et al. The International Scientific Association of Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of postbiotics. Nature Reviews Gastroenterology & Hepatology. 2021. vol. 18. no. 9. pp. 649-667.
17. Thomas L.V. Probiotics–the journey continues. International Journal of Dairy Technology. 2016. vol. 69. no. 4. pp. 469-480. doi: 10.1111/1471-0307.12354
18. Uyeno Y., Shigemori S., Shimosato T. Effect of probiotics/prebiotics on cattle health and productivity. Microbes and environments. 2015. vol. 30. no. 2. pp. 126-132. doi: 10.1264/jsme2.ME14176
19. Shokryazdan P., Faseleh Jahromi M., Liang J.B., Ho Y.W. Probiotics: from isolation to application. Journal of the American College of Nutrition. 2017. vol. 36. no. 8. pp. 666-676. doi: 10.1080/07315724.2017.1337529
20. Alayande K.A., Aiyegoro O.A., Ateba C.N. Probiotics in animal husbandry: Applicability and associated risk factors. Sustainability. 2020. vol. 12. no. 3. pp. 1087.
Review
For citations:
Volkova G.S. Establishment of probiotic associations for foods and feeds. Proceedings of the Voronezh State University of Engineering Technologies. 2024;86(1):103-107. (In Russ.) https://doi.org/10.20914/2310-1202-2024-1-103-107
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