Electrophysical effect on protein raw materials
https://doi.org/10.20914/2310-1202-2024-2-113-118
Abstract
Organic polymers are used in all aspects of human life and are now recognized as important materials for society. To develop organic polymer materials, it is necessary to understand the structure of the polymer. Proteins are essential components of living matter belonging to the class of polyelectrolytes. They are the main polymers among organic substances and have a special complexity of structure. Protein macromolecules also have high mobility under changing environmental conditions. This makes it easier to create organic substances with new properties. Casein is a complex protein with a powerful amino acid composition. The results of experiments on the development of methods for changing the isoelectric point of milk casein protein in two accessible and technologically simple ways are presented. The first method is based on the use of water with an admixture of a modern nanomaterial - fullerene, which is a new allotropic modification of carbon isolated from the natural material shungite. A significant shift of the isoelectric point of casein to the alkaline region was noted, the mechanism of the observed phenomenon was proposed, and its practical significance for improving some technological stages in food production was determined. It was found that casein coagulation (IETB) was observed at pH =5.8 (the standard value of the IETB region is pH =4.6–4.7). The second method is based on the use of water pretreated with microwaves with a frequency of 2.45 GHz. The shift of the isoelectric point to the acidic side has been established. In the conducted tests, the positive charge in the electrostatic balance of macromolecules decreases and a reduced (pH = 3.2) value of IETB is observed. The mechanism of the observed phenomenon is proposed, the practical significance of the found technique for the production of whole milk products with an extended shelf life is determined.
About the Authors
Y. V. UstinovaRussian Federation
Cand. Sci. (Engin.), associate professor, technology of storage and processing of animal products department, st. Timiryazevskaya, 4, Moscow, 127434, Russia
D. M. Borodulin
Dr. Sci. (Eng.), professor, technology of storage and processing of animal products department, st. Timiryazevskaya, 4, Moscow, 127434, Russia
References
1. Vikhareva I.N., Zaripov I.I., Kinzyabulatova D.F. et al. Biodegradable polymer materials and modifying additives: current state. Part 1. Nanotechnologies in construction: scientific online journal. 2020. vol. 12. no. 6. pp. 320–325. (in Russian).
2. Chang C.I.D., Zenitova L.A. Polymer composite material based on polyurethane foam and chitin and its properties. Bulletin of the Technological University. 2021. vol. 24. no. 2. pp. 56–60. (in Russian).
3. Leskova S.A. Problems of biodegradation of polyolefins using the example of polyethylene. Innovations. The science. Education. 2021. no. 40. pp. 309–315. (in Russian).
4. Nguyen Ch.N., Pykhtin A.A., Simonov-Emelyanov I.D. Dispersed deformable particles, calculation of compositions and technology for producing highly filled polymer composite materials. Plastic masses. 2022. no. 5–6. pp. 39–44. (in Russian).
5. Dolinskaya R.M., Prokopchuk N.R. The use of crumb rubber as a filler for thermoplastics (review). Proceedings of BSTU. Series 2: Chemical technologies, biotechnology, geoecology. 2022. no. 1 (253). pp. 37–44. (in Russian).
6. Khudyakova N., Stupina A., Klassen I. Frequency of occurrence of beta-casein gene alleles in different breeds of cattle. Agricultural Scientific Journal. 2023. pp. 85–91. doi: 10.28983/asj.y2023i4pp85–91 (in Russian).
7. Melnikova E.I., Stanislavskaya E.B., Bogdanova E.V., Shabalova E.D. Features of the production and use of micellar casein in the technology of milk-intensive protein products. Equipment and technology of food production. 2022. vol. 52. no. 3. pp. 592–601. (in Russian).
8. Sentsova T.B., Ilyenko L.I., Kazyukova T.V. and others. Comparative nutritional effectiveness of casein-dominant and whey formulas used for feeding children in the first half of life. Pediatrics. Journal named after G.N. Speransky. 2019. vol. 98. no. 4. pp. 149–157. (in Russian).
9. Fedorov A.A., Sochivko D.G., Varlamov D.A., Kurochkin V.E. Model of linear inhibition of enzyme activity during the polymerase chain reaction. Journal of Technical Physics. 2022. vol. 92. no. 7. pp. 958–962. (in Russian).
10. Chen G.Q., Qu Y., Gras S.L., Kentish S.E. Separation technologies for whey protein fractionation. Food Engineering Reviews. 2023. vol. 15. no. 3. pp. 438-465. doi: 10.1007/s12393–022–09330–2
11. Pedrali D., Scarafoni A., Giorgi A., Lavelli V. Binary Alginate-Whey Protein Hydrogels for Antioxidant Encapsulation. Antioxidants. 2023. vol. 12. no. 6. pp. 1192. doi: 10.3390/antiox12061192
12. Miwa K., Aoyagi S., Sasamori T., Morisako S. et al. Facile Multiple Alkylations of C60 Fullerene. Molecules. 2022. vol. 27. no. 2. pp. 450.
13. Tuktarov A.R., Khuzin A.A., Dzhemilev U.M. Fullerene-containing lubricants: Achievements and prospects. Petroleum Chemistry. 2020. vol. 60. pp. 113-133. doi: 10.1134/S0965544120010144
14. Fernandes N.B., Shenoy R.U.K., Kajampady M.K., DCruz C.E. et al. Fullerenes for the treatment of cancer: an emerging tool. Environmental Science and Pollution Research. 2022. vol. 29. no. 39. p. 58607-58627. doi: 10.1007/s11356-022-21449-7
15. Yamaguchi N., Sano H., Sawahata H., Nakano M. et al. Statistical analysis of properties of non-fullerene acceptors for organic photovoltaics. Japanese Journal of Applied Physics. 2022. vol. 61. no. 3. pp. 030905. doi: 10.35848/1347-4065/ac4894
16. Mumtaz S., Rana J.N., Choi E.H., Han I. Microwave radiation and the brain: Mechanisms, current status, and future prospects. International Journal of Molecular Sciences. 2022. vol. 23. no. 16. pp. 9288.
17. Al_Dulamey Q.K. The Development of microwave applications in medical field. Rafidain Journal of Science. 2021. vol. 30. no. 2. pp. 23-39.
18. Chan J.H., Mumtaz S., Lee S.V., Kim, D.–Y. et al. Focusing high-power microwaves with positive and negative band plates to increase the receiving power in an axial axial oscillator with a virtual cathode. Curr. Appl. Phys. 2021. vol. 29. pp. 89–96.
19. Ryan T.P. History and development of microwave thermal therapy. Principles and Technologies for Electromagnetic Energy Based Therapies. Academic Press, 2022. pp. 313-347.
20. Mumtaz S., Rana J.N., Choi E.H., Han I. Microwave radiation and the brain: Mechanisms, current status, and future prospects. International Journal of Molecular Sciences. 2022. vol. 23. no. 16. pp. 9288. doi: 10.3390/ijms23169288
21. Balan D.D., Kulemin I.V. Experimental study of the properties of microwave radiation. Step into science. 2023. no. 1. pp. 11–16. (in Russian).
22. Avakyan S.V., Baranova L.A. Microwave radiation in the problem of modern viral diseases. Bulletin of the Russian Academy of Sciences. 2022. vol. 92. no. 4. pp. 372–383. (in Russian).
23. Petrova S.Yu., Khlgatyan S.V., Emelyanova O.Yu. et al. Modern information about milk caseins. Bioorganic chemistry. 2022. vol. 48. no. 2. pp. 207–216. (in Russian).
24. Shevchenko T.V., Dubinina I.E., Ustinova Yu.V., Popov A.M. Production of colored food starches. Storage and processing of agricultural raw materials. 2018. no. 4. pp. 75–83. (in Russian).
25. Senchenkova E.A., Borovskaya L.V. The process of coagulation of milk proteins. The Scientific Heritage. 2021. no. 80–3. pp. 28–31. (in Russian).
26. Shevchenko T.V., Ustinova Yu.V., Popov A.M. Method for changing the isoelectric point of a protein using activated water. Patent RF, no. 2794151, 2023.
27. Shevchenko T.V., Ustinova Yu.V., Yustratov V.P., Bezrukov M.S. and others. Use of fullerene during storage and drying of apples. Storage and processing of agricultural raw materials. 2020. no. 2. pp. 85-93. (in Russian).
28. Titorenko E., Ermolaeva E., Ivanov P., Ustinova Yu. Designing the technology and composition of plant extracts using reduced atmospheric pressure. Nexo Revista Científica. 2023. vol. 36. no. 02. pp. 139–147.
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Review
For citations:
Ustinova Y.V., Borodulin D.M. Electrophysical effect on protein raw materials. Proceedings of the Voronezh State University of Engineering Technologies. 2024;86(2):113-118. (In Russ.) https://doi.org/10.20914/2310-1202-2024-2-113-118