Optimization of ultrasound aqueous extraction of phenolic compounds from sea buckthorn berries (Hippophae rhamnoides L.) using Box-Behnken design
https://doi.org/10.20914/2310-1202-2026-1-176-183
Abstract
Sea buckthorn (Hippophae rhamnoides L.) is a valuable source of bioactive compounds, including phenolic compounds with pronounced antioxidant activity. The aim of this study was to optimize ultrasound-assisted aqueous extraction parameters from sea buckthorn berries to maximize phenolic compounds yield and antioxidant activity using Box-Behnken experimental design. Extraction temperature (50–90 °C), ultrasonic treatment time with sonotrode (0–5 min within 45 min total extraction time), and liquid-to-solid ratio (1:10–1:30) were selected as independent variables. Three responses were evaluated: free radical scavenging activity FRSA (%), total phenolic content TPS by Folin-Ciocalteu method, and dry matter content DM (%). Fifteen experiments were conducted, including three replicates at the center point. Second-order mathematical models with high determination coefficients were developed (R² = 0.898 for IC50, R² = 0.983 for TPC, R² = 0.889 for DM). It was established that extraction temperature has a significant positive effect on IC50 (-2,45), and TPC (+3.74). As for liquid-to-solid ratio – negative effect (-1,11). Ultrasound treatment time had no statistically significant effect on all studied responses. Optimal parameters were determined: for IC50 miniization – temperature 90 °C, liquid-to-solid ratio 1:12–15 (predicted FRSA ≈ 5,4–7,6); for TPC maximization – temperature 50 °C, liquid-to-solid ratio 1:30 (predicted TPS ≈ 22 mg GAE/g). The developed models can be applied for industrial production of sea buckthorn extracts with specified properties
About the Authors
M. S. Ivanov
ITMO University
Postgraduate student, Faculty of Biotechnology, Lomonosova street 9, Saint Petersburg, 192002, Russia
N. V. Iakovchenko
ITMO University
Cand. Sci. (Engin.), Faculty of Biotechnology, Lomonosova street 9, Saint Petersburg, 192002, Russia
E. V. Kravtcova
ITMO University
Cand. Sci. (Engin.), Faculty of Biotechnology, Lomonosova street 9, Saint Petersburg, 192002, Russia
V. A. Shiriaev
ITMO University
Postgraduate student, Faculty of Biotechnology, Lomonosova street 9, Saint Petersburg, 192002, Russia
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For citations:
Ivanov M.S., Iakovchenko N.V., Kravtcova E.V., Shiriaev V.A. Optimization of ultrasound aqueous extraction of phenolic compounds from sea buckthorn berries (Hippophae rhamnoides L.) using Box-Behnken design. Proceedings of the Voronezh State University of Engineering Technologies. 2026;88(1):176-183. (In Russ.) https://doi.org/10.20914/2310-1202-2026-1-176-183
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