Biotech platform for the production of protein concentrate from beans
https://doi.org/10.20914/2310-1202-2026-1-213-218
Abstract
This study highlights the task of creating resource-efficient methods for the production of protein preparations from plant raw materials. It is emphasized that beans, which have significant protein potential, still do not have well-developed technologies for processing into isolates and concentrates. The aim of the work was to develop and optimize an environmentally friendly biotechnological platform for the production of bean protein concentrate using enzymatic degradation of ballast polysaccharides. During the research, the fractional composition of bean flour proteins was analyzed, a rational selection of enzyme preparations (amylosubtilin G10x and glucavamorin G20x) was made, and key parameters of the hydrolysis process (hydromodule, temperature, pH level, duration and ratio of enzymes) were optimized. It was found that the maximum level of starch hydrolysis (76%) is achieved when using an enzyme composition in a ratio of 65:35, a dosage of 2.5 units / g of starch, a temperature of 40 °C, pH 4.6 and a process duration of 4 hours. An integrated technological scheme has been developed, including the stages of fermentation, protein sedimentation at an isoelectric point, washing and freeze drying. The yield of the target product was 65.5% of the initial protein content in the raw material. The proposed technology is characterized by reduced energy consumption, the exclusion of the use of aggressive chemicals and the possibility of recycling recycled products.
About the Authors
V. I. Manzhesov
Voronezh State Agrarian University named after Emperor Peter the Great
Russian Federation
Russian Federation
S. Y. Churikova
Voronezh State Agrarian University named after Emperor Peter the Great
Russian Federation
Russian Federation
A. A. Hayrapetyan
Voronezh State Agrarian University named after Emperor Peter the Great
Russian Federation
Russian Federation
S. F. Yakovleva
Voronezh State University of Engineering Technologies
Russian Federation
Russian Federation
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For citations:
Manzhesov V.I., Churikova S.Y., Hayrapetyan A.A., Yakovleva S.F. Biotech platform for the production of protein concentrate from beans. Proceedings of the Voronezh State University of Engineering Technologies. 2026;88(1):213-218. (In Russ.) https://doi.org/10.20914/2310-1202-2026-1-213-218
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