Comparative analysis (review) of foaming ability of vegetable and animal proteins for use in food systems
https://doi.org/10.20914/2310-1202-2026-2-
Abstract
The article presents an analysis of the scientific literature, which indicates the significant potential of various types of flour and non-traditional sources as protein foaming agents for use in food systems. The most promising from the point of view of foaming are rye flour, aquafaba, pumpkin seed flour after microfluidization and soy isolate, which exhibit high foaming ability, and rye flour and flax protein concentrate also form very stable foams. A comparative analysis of the foaming properties of protein components that make up flour from traditional cereals (wheat, rye, corn, oats, rice), legumes (soybeans, peas, chickpeas, lentils), pseudo-grain (buckwheat, amaranth) crops, as well as non-traditional protein sources (insects, fenugreek, secondary wheat products). The molecular mechanisms of formation and stabilization of food foams, the determining role of water-soluble fractions (albumins) and the influence of protein-polysaccharide complexes are considered. The data on the effects of technological factors (pH, temperature, protein concentration) and physico-chemical modification (enzymatic hydrolysis, microfluidization, radiofrequency heating) are systematized the foaming ability and stability of foams. It has been established that rye flour, aquafaba and soy isolate have the greatest potential as effective foaming agents, combining high foaming ability with good foam stability. Special attention is paid to the comparative characteristics of the behavior of proteins at the interface of the "air-water" and "oil-water" phases, as well as the prospects for using combined protein systems and extracts (aquafabs) to create products with specified rheological properties and increased nutritional value. The results of the review are of practical importance for use in food systems, in particular bakery technology.
About the Authors
E. M. KolodinaCand. Sci. (Engin.), lecturer, food technology and catering department, Pervomaiskaya str., 191, Maykop, Republic of Adygea, 385000, Russia
K. E. Syrykh
2-year master's student in the field of training: 19.04.04 Technology of products and catering, food technology and catering department, Pervomaiskaya str., 191, Maykop, Republic of Adygea, 385000, Russia
G. O. Magomedov
Dr. Sci. (Engin.), professor, Bakery technology, confectionery, pasta and grain processing industries department, Revolution Av., 19 Voronezh, 394036, Russia
M. G. Magomedov
Dr. Sci. (Engin.), professor, Bakery technology, confectionery, pasta and grain processing industries department, Revolution Av., 19 Voronezh, 394036, Russia
Z. N. Khatko
Dr. Sci. (Engin.), professor, head of the department, food technology and catering department, Pervomaiskaya str., 191, Maykop, Republic of Adygea, 385000, Russia
References
1. Oduse K., Arogundade L.A., Deng Y. et al. Electrostatic complexes of whey protein and pectin as foaming and emulsifying agents. International Journal of Food Properties. 2020. vol. 20. no. sup3. pp. S3027–S3041. doi: 10.1080/10942912.2017.1396478.
2. Kulikov D.S., Korolev A.A., Pankratov I.V. Analysis of modern methods for obtaining hydrolysates and bioactive peptides from wheat protein components. Food Industry. 2025. no. 4. pp. 88–94. doi: 10.52653/PPI.2025.4.4.016 (in Russian).
3. Barros J.H.T., de Souza C.K., Telis-Romero J. et al. Non-thermal emerging technologies as alternatives to chemical additives to improve the quality of wheat flour for breadmaking: a review. Critical Reviews in Food Science and Nutrition. 2023. vol. 63. no. 11. pp. 1612–1628. doi: 10.1080/10408398.2021.1966735.
4. Kuznetsova L.I., Burykina M.S., Parakhina O.I., Dutchina M.A. Analysis of the quality of rye wholemeal flour produced by flour mills in various regions of Russia in 2020. Baking in Russia. 2021. no. 2. pp. 36–43. (in Russian).
5. Ma H.F., Wu W., Chen L. et al. Structural and functional properties of soy protein isolates from different cultivars. International Journal of Biological Macromolecules. 2025. vol. 300. article 146748. doi: 10.1016/j.ijbiomac.2025.146748.
6. Dwivedi S.K., Issar K., Tiwari V. Nutrient requirements in health and disease. In: Handbook of Nutraceuticals: Science, Technology and Engineering. Cham: Springer Nature Switzerland, 2026. pp. 151–175. doi: 10.1007/978-3-031-65467-5_7.
7. Echeverria-Jaramillo E., Jaramillo-García J.D., Fernández-López J. et al. Functional properties of protein fractions from gentle membrane separation of green biomass (legume grass) compared to traditional animal-and plant-based proteins. Future Foods. 2025. vol. 11. article 100740. doi: 10.1016/j.fufo.2025.100740.
8. Ikram A., Saeed F., Afzaal M. et al. A comprehensive review on biochemical and technological properties of rye (Secale cereale L.). International Journal of Food Properties. 2023. vol. 26. no. 1. pp. 2212–2228. doi: 10.1080/10942912.2023.2236321.
9. Popov V.N., Plotnikova I.V., Magomedov G.O. et al. Comprehensive assessment of the foaming properties of whey protein concentrate for the production of special purpose products. Food Industry. 2020. no. 8. pp. 42–47. doi: 10.24411/0235-2486-2020-10084 (in Russian).
10. Zhang S., Liu Y., Wu W. Study on the structural characteristics and foaming properties of ovalbumin – Citrus pectin conjugates prepared by the Maillard reaction. Foods. 2024. vol. 13. no. 22. article 3542. doi: 10.3390/foods13223542.
11. Stamatie G.D., Zaharia R., Tofană M. et al. Nutritional and functional properties of some protein sources. AgroLife Scientific Journal. 2021. vol. 10. no. 1. pp. 185–192.
12. Premkumar J., Ravi R., Roy S. et al. Corn protein isolate: Characteristic analysis, functional properties, and utilization in beverage formulation. Journal of Food Processing and Preservation. 2022. vol. 46. no. 2. article e16257. doi: 10.1111/jfpp.16257.
13. Yakovleva A.A. Prospects for the use of hemp and flax seeds as a source of food protein. Technical Crops. Scientific Agricultural Journal. 2024. no. 2(4). pp. 3–9. (in Russian).
14. Qazanfarzadeh Z., Kadivar M., Shekarchizadeh H. et al. Functional properties of rye prolamin (Secalin) and their improvement by protein lipophilization through capric acid covalent binding. Foods. 2021. vol. 10. no. 3. article 515. doi: 10.3390/foods10030515.
15. Janssen F., Monterde V., Wouters A.G.B. Relevance of the air–water interfacial and foaming properties of (modified) wheat proteins for food systems. Comprehensive Reviews in Food Science and Food Safety. 2023. vol. 22. no. 3. pp. 1517–1554. doi: 10.1111/1541-4337.13147.
16. Zhao M., Lan Y., Zhang H. et al. Comparative study on the foam and air-water interface properties of ethanol-soluble and non-ethanol components in wheat aqueous phase protein. Food Hydrocolloids. 2024. vol. 150. article 109700. doi: 10.1016/j.foodhyd.2024.109700.
17. Almasova M.G., Tedeeva F.L. Influence of wheat germ on the structural and mechanical properties of dough and the quality of finished bread. In: Days of Science of NOSU 2023: Materials of the scientific conference based on the results of the work of the Faculty of Chemistry, Biology and Biotechnology of NOSU, Vladikavkaz, April 23, 2024. Vladikavkaz: NOSU, 2024. pp. 18–31. (in Russian).
18. Oyeyinka S.A., Bassey I.A.V. Composition, functionality, and baking quality of flour from four brands of wheat flour. Journal of Culinary Science & Technology. 2025. vol. 23. no. 1. pp. 87–107. doi: 10.1080/15428052.2023.2254821.
19. Govyadova I.A., Starkova A.V., Kovaleva A.E., Pyanikova E.A. Development of recipe and technology for the production of yeast-free whole grain bread. In: Food Industry in Modern Conditions: Trends and Innovations: Collection of scientific articles of the International Scientific and Practical Conference, Orel, April 19, 2023. Orel: Orel State Agrarian University, 2023. pp. 53–58. (in Russian).
20. Zhang X., Xu Y., Li J. et al. Interfacial and foaming properties of plant and microbial proteins: Comparison of structure-function behavior of different proteins. Food Chemistry. 2025. vol. 463. article 141431. doi: 10.1016/j.foodchem.2024.141431.
21. Bochkareva Z.A., Pchelintseva O.N., Belyakova K.N., Sagandykova S.K. Comparative assessment of indicators of rye bread on spontaneous fermentation sourdoughs. Polzunovsky Bulletin. 2022. no. 1. pp. 23–30. doi: 10.25712/ASTU.2072-8921.2022.01.003 (in Russian).
22. Iuga M., Mironeasa S., Codină G.G. et al. Impact of dairy ingredients on wheat flour dough rheology and bread properties. Foods. 2020. vol. 9. no. 6. article 828. doi: 10.3390/foods9060828.
23. Shevchuk E.V., Dolgakova M.A. Study of the influence of preheating of amaranth, corn and rice flour on the foaming properties of raw egg white. In: Technique and Technology of Food Production: Abstracts of the XI International Scientific Conference. Mogilev: MSUF, 2019. p. 130. (in Russian).
24. Burykina M.S., Kuznetsova L.I., Dutchina M.A. Study of the relationship between the amount of protein in rye and wheat flour and its technological properties. Viticulture and Winemaking. 2023. vol. 52. pp. 69–71. (in Russian).
25. Kuznetsova L.I., Burykina M.S., Savkina O.A. et al. Study of the quality of rye wholemeal flour and its foaming capacity. Baking in Russia. 2022. no. 1. pp. 47–50. doi: 10.37443/2073-3569-2022-1-1-47-50 (in Russian).
26. Vani B., Zayas J.F. Foaming properties of selected plant and animal proteins. Journal of Food Science. 1995. vol. 60. no. 5. pp. 1025–1028. doi: 10.1111/j.1365-2621.1995.tb06293.x.
27. Janssen F., Pauly A., Rombouts I. et al. The role of non-starch polysaccharides in determining the air-water interfacial properties of wheat, rye, and oat dough liquor constituents. Food Hydrocolloids. 2020. vol. 105. article 105771. doi: 10.1016/j.foodhyd.2020.105771.
28. Derkanosova N.M., Stakhurlova A.A., Vasilenko O.A. Prediction of the quality of bakery products based on the study of rheological properties of model mixtures. Commodity Expert of Food Products. 2024. no. 7. pp. 404–407. doi: 10.33920/igt-01-2407-04 (in Russian).
29. Song M.K., Guo X.N., Zhu K.X. Alkali-induced protein structural, foaming, and air–water interfacial property changes and quantitative proteomic analysis of buckwheat sourdough liquor. Journal of Agricultural and Food Chemistry. 2024. vol. 72. no. 27. pp. 15387–15397. doi: 10.1021/acs.jafc.4c03210.
30. Ninomiya K., Abe Y., Matsumura Y. et al. Physicochemical and functional properties of buckwheat (Fagopyrum esculentum Moench) albumin. Future Foods. 2022. vol. 6. article 100178. doi: 10.1016/j.fufo.2022.100178.
31. Song M.K., Guo X.N., Zhu K.X. Elucidating the gas cell stabilization mechanism of buckwheat-wheat steamed bread induced by transglutaminase: A focus on the foaming and air-water interfacial properties of dough liquor. Food Hydrocolloids. 2025. vol. 159. article 110701. doi: 10.1016/j.foodhyd.2024.110701.
32. Li T., Wang L., Zhang X. et al. Formation, structural characteristics, foaming and emulsifying properties of rice glutelin fibrils. Food Chemistry. 2021. vol. 354. article 129554. doi: 10.1016/j.foodchem.2021.129554.
33. Jiang F., Li M., Wang Y. et al. Self-assembly of rice proteins: A perspective on elevating rice protein techno-functional properties. Trends in Food Science & Technology. 2024. vol. 151. article 104624. doi: 10.1016/j.tifs.2024.104624.
34. Wang D., Li X., Zhang Y. et al. Modification of rice protein and its components: Enhanced fibrils formation and improved foaming properties. Food Hydrocolloids. 2025. vol. 158. article 110575. doi: 10.1016/j.foodhyd.2024.110575.
35. Li D., Zhao Y., Wang R. et al. Changes of structure and functional properties of rice protein in the fresh edible rice during the seed development. Food Science and Human Wellness. 2023. vol. 12. no. 5. pp. 1850–1860. doi: 10.1016/j.fshw.2023.02.034.
36. Stone A.K., Nosworthy M.G., Chiremba C., House J.D., Nickerson M.T. A comparative study of the functionality and protein quality of a variety of legume and cereal flours. Cereal Chemistry. 2019. vol. 96. pp. 1159–1169. doi: 10.1002/cche.10237.
37. Urubkov S.A., Korolev A.A., Smirnov S.O. Ways to increase the bioavailability of legume raw materials in the technology of instant food concentrates. Modern Science and Innovation. 2019. no. 3 (27). pp. 111–118. (in Russian).
38. Kumar S., Brooks M.S.-L. Enrichment and recovery of pea (Pisum sativum L.) proteins using foam fractionation for simultaneous enhancement of their functional properties. Separation and Purification Technology. 2025. vol. 364. pt 3. article 132578. doi: 10.1016/j.seppur.2025.132578.
39. Jarpa-Parra M., Bamdad F., Wang Y. et al. Understanding the stability mechanisms of lentil legumin-like protein and polysaccharide foams. Food Hydrocolloids. 2019. vol. 61. pp. 903–913. doi: 10.1016/j.foodhyd.2016.06.026.
40. Khan M.J., Chaudhary S., Ali A., Manickavasagam A. Enzyme-assisted extraction of navy bean protein from whole and dehulled flour: Effects on extractability, structure, and techno-functional properties. International Journal of Biological Macromolecules. 2026. vol. 337. pt 1. article 149476. doi: 10.1016/j.ijbiomac.2026.149476.
41. Lafarga T., Álvarez C., Villaró S., Bobo G., Aguiló-Aguayo I. Potential of pulse derived proteins for developing novel vegan edible foams and emulsions. International Journal of Food Science & Technology. 2020. vol. 55. article 14286. doi: 10.1111/ijfs.14286.
42. Yang J., Wang C., Li Y. et al. Physical, interfacial and foaming properties of different mung bean protein fractions. Food Hydrocolloids. 2023. vol. 143. article 108885. doi: 10.1016/j.foodhyd.2023.108885.
43. Vitol I.S., Pankratov G.N., Meleshkina E.P. Biochemical characteristics of new varieties of flour from a binary mixture of wheat and flax. IOP Conference Series: Earth and Environmental Science. 2021. vol. 640. no. 2. article 022050. doi: 10.1088/1755-1315/640/2/022050.
44. Yan X., Zhu F. Structural, physicochemical and functional properties of quinoa, buckwheat and amaranth protein isolates: A comparative study. Food Chemistry. 2025. vol. 491. article 145301. doi: 10.1016/j.foodchem.2025.145301.
45. Nooshkam M., Varidi M., Alkobeisi F. Bioactive food foams stabilized by licorice extract/whey protein isolate/sodium alginate ternary complexes. Food Hydrocolloids. 2022. vol. 126. article 107488. doi: 10.1016/j.foodhyd.2022.107488.
46. Garmash N.Yu., Cherevach E.I., Levochkina L.V., Zubova V.V. Prospects for the use of non-traditional vegetable foaming agents in the technology of sweet desserts for functional purposes. In: Modern Problems of Commodity Science, Economics and Food Industry: Collection of articles based on the results of the I Correspondence International Scientific and Practical Conference, Saratov, November 30, 2016. Saratov: SSEI of Plekhanov Russian University of Economics, 2019. pp. 72–76. (in Russian).
47. Sumina S.R., Merkuryev N.V. Analysis and comparative characteristics of the quality of spelt flour from different manufacturers. In: Safety and Quality of Agricultural Raw Materials and Food 2023: Proceedings of the All-Russian Scientific and Practical Conference, Moscow, November 22–23, 2023. Moscow: Sam Poligrafist, 2023. pp. 507–511. (in Russian).
48. Nikonorova Yu.Yu., Volkova A.V., Makushin A.N. Study of the rheological properties of dough and bread from a mixture of premium wheat flour and sorghum flour. Bulletin of KrasGAU. 2021. no. 4(169). pp. 155–160. doi: 10.36718/1819-4036-2021-4-155-160 (in Russian).
49. Nikonorova Yu.Yu., Volkova A.V. Influence of the use of flour from amaranth, sorghum and millet grains on the processes of fermentation and dough ripening. Eurasian Union of Scientists. 2020. no. 7-8 (76). pp. 31–35. (in Russian).
50. Nigmatzyanov A.S., Koshchina E.I., Nikulina N.Sh., Zagranichnaya A.D. The use of amaranth flour in the production of bakery products. In: Agro-Industrial Complex of Russia: Education, Science, Production: Collection of articles of the VII All-Russian (national) Scientific and Practical Conference with international participation, Saratov, December 19–21, 2023. Penza: Penza State Agrarian University, 2024. pp. 121–126. (in Russian).
51. Badamshina E.V., Gareeva I.T., Leonova S.A., Koshchina E.I. Influence of triticale flour and bran on the quality indicators of bread sticks. Bulletin of KrasGAU. 2023. no. 11(200). pp. 298–304. doi: 10.36718/1819-4036-2023-11-298-304 (in Russian).
52. Belyaev A.G., Kaluzhskikh A.G., Boev S.G., Cherkasina A.A. Study of the possibility of using fireweed and peanut flour products in the technology of rye-wheat bread on rye sourdough. In: Commodity Science, Technology and Expertise: Innovative Solutions and Development Prospects: Proceedings of the National Scientific and Practical Conference, Moscow, October 28, 2020. Moscow: ZooVetKniga, 2020. pp. 119–125. (in Russian).
53. Rusina I.M., Kalesnik I.M. Investigation of quality indicators of bread sticks based on first grade wheat flour, rye flour and table beet powder. Modern Science and Innovations. 2022. no. 2(38). pp. 62–70. doi: 10.37493/2307-910X.2022.2.6.
54. Kukarkina Yu.K., Makhiyanova D.N., Gabdukaeva L.Z. Different types of flour from non-traditional sources of raw materials to expand the range of flour products. In: Food Industry in Modern Conditions: Trends and Innovations: Collection of scientific articles of the International Scientific and Practical Conference, Orel, April 19, 2023. Orel: Orel State Agrarian University, 2023. pp. 252–255. (in Russian).
55. Ostermann-Porcel M.V., Rinaldoni A.N., Campderrós M.E. Assessment of Jerusalem artichoke as a source for the production of gluten-free flour and fructan concentrate by ultrafiltration. Applied Food Research. 2022. vol. 2. no. 2. article 100201. doi: 10.1016/j.afres.2022.100201.
56. Pilyakina V.D., Derkanosova N.M., Stakhurlova A.A., Vasilenko O.A. Study of the effect of lupine on the baking properties of flour model mixtures. In: International Scientific and Practical Conference Dedicated to the Memory of Vasily Matveevich Gorbatov. 2024. no. 1. pp. 123–128. (in Russian).
57. Ghanghas N., Prabhakar P.K., Sharma S., Mukilan M.T. Microfluidization of fenugreek (Trigonella foenum graecum) seed protein concentrate: Effects on functional, rheological, thermal and microstructural properties. LWT. 2021. vol. 149. article 111830. doi: 10.1016/j.lwt.2021.111830.
58. Devyatkin D.I., Chugunova O.V., Pankratyeva N.A. Production of amaranth meal protein hydrolysate with enhanced technological properties. In: Modern Achievements of Biotechnology: Vector for Technological Leadership: Proceedings of the X International Scientific and Practical Conference, Stavropol, October 21–25, 2025. Stavropol: Bureau of News, 2025. pp. 93–96. (in Russian).
59. Krasnoshtanova A.A., Shults L.V. Production and evaluation of functional properties of protein isolates and hydrolysates from plant raw materials. Chemistry of Plant Raw Materials. 2022. no. 4. pp. 299–309. doi: 10.14258/jcprm.20220410952 (in Russian).
60. Siddiqui S.A., Singh S., Bahmid N.A. et al. Unveiling the diversity of Non-conventional Proteins-From sources, extraction, technofunctionality, nutraceutical potential to advancement in food Applications-A systematic review. Waste and Biomass Valorization. 2025. vol. 16. no. 1. pp. 29–51. doi: 10.1007/s12649-024-02615-5.
61. Yisa Njowe K.B., Duodu K.G., Emmambux M.N. Techno-functional properties of protein-rich flours from different species of edible insects as affected by drying methods. Food Research International. 2025. vol. 221. pt 1. article 117224. doi: 10.1016/j.foodres.2025.117224.
62. Basangova N.G., Fedinishina E.Yu. Study of the technological properties of aquafaba from various types of legumes. In: Biotechnology and Safety in the Technosphere: Collection of materials of the All-Russian Conference, Saint Petersburg, March 2–3, 2022. Saint Petersburg: SPbPU, 2022. pp. 145–147. (in Russian).
63. Romanova Kh.S., Strizhevskaya V.N., Simakova I.V. et al. Study of the structure-forming properties of aquafaba from red and white beans. Technologies of Food and Processing Industry of the Agro-Industrial Complex – Healthy Food Products. 2025. no. 2. pp. 50–58. doi: 10.24412/2311-6447-2025-2-50-58 (in Russian).
64. Plotnikova I.V., Magomedov G.O., Shevyakova T.A. et al. The use of lentil bean suspension in the production of muffins for lenten and vegetarian nutrition. Khleboprodukty. 2020. no. 6. pp. 38–41. doi: 10.32462/0235-2508-2020-29-6-38-41 (in Russian).
65. Patent no. 2737670 C1, Russian Federation, IPC A23G 3/36, A23G 3/52. Method for producing marshmallow without egg white. Plotnikova I.V., Magomedov G.O., Gubkovskaya V.V. et al.; applicant and patent holder Voronezh State University of Engineering Technologies. no. 2020111370; filed 19.03.2020; publ. 02.12.2020. (in Russian).
66. Plotnikova I.V., Magomedov G.O., Polyansky K.K. et al. Study of the functional properties of whey protein concentrate for its further use in the confectionery industry. In: Food Technologies of the Future: Innovative Ideas, Scientific Search, Creative Solutions: Collection of materials of the Scientific and Practical Youth Conference dedicated to the memory of R.D. Polandova, Moscow, June 5, 2020. Moscow: Buki Vedi, 2020. pp. 180–184. (in Russian).
67. Voskanyan O.S., Shipilova P.A. Development of a new type of ice cream with Crimean spices based on aquafaba. Bulletin of Razumovsky Moscow State University of Technology and Management. Series of Applied Scientific Disciplines. 2023. no. 1. pp. 23–32. (in Russian).
68. Tarasov A.V., Zavorokhina N.V., Chugunova O.V., Vyatkin A.V. Correlation of foaming properties of plant-based beverages with physicochemical composition. Food Systems. 2025. vol. 8. no. 2. pp. 306–312. doi: 10.21323/2618-9771-2025-8-2-306-312 (in Russian).
69. Siddiqui S.A., Asif Z., Murid M. et al. New alternatives from sustainable sources to wheat in bakery foods: Science, technology, and challenges. Journal of Food Biochemistry. 2022. vol. 46. no. 9. article e14185. doi: 10.1111/jfbc.14185.
70. Mirzaee H., Razavi S.H., Khodaiyan F. et al. Improved antioxidant, antihypertensive, and antidiabetic activities and tailored emulsion stability and foaming properties of mixture of corn gluten and soy protein hydrolysates via enzymatic processing and fractionation. Food Science & Nutrition. 2024. vol. 12. no. 11. pp. 9749–9763. doi: 10.1002/fsn3.3421.
71. Chen J., Ozturk O.K. Structure-function modulation of protein-rich pumpkin seed flour via microfluidization processing for plant-based applications. Food Chemistry. 2026. vol. 499. article 147416. doi: 10.1016/j.foodchem.2026.147416.
72. Hu D., Yang G., Tian Y. et al. Effect of radio frequency heating on structure and physicochemical properties of protein and starch based on gelatinization degree of rice flour. Food Research International. 2025. vol. 218. article 116902. doi: 10.1016/j.foodres.2025.116902.
73.
Review
For citations:
Kolodina E.M., Syrykh K.E., Magomedov G.O., Magomedov M.G., Khatko Z.N. Comparative analysis (review) of foaming ability of vegetable and animal proteins for use in food systems. Proceedings of the Voronezh State University of Engineering Technologies. 2026;88(2):172-186. (In Russ.) https://doi.org/10.20914/2310-1202-2026-2-
JATS XML



























