The process of biotransformation containing sugar raw isomaltulosesynthase bacteria of the genus Erwinia to produce isomaltulose - natural sugars substitute. Raw cane sugar, beet molasses and sweet sorghum syrup used for isomaltulose. It was established that cane sugar may serve as a substrate for the enzymatic reaction together with biotransformation of pure sucrose. Yield of isomaltulose in the transformation of raw sugar was comparable to the control and was 97% for 3,5 hours at the optimal isomerization conditions (pH 6,0, 30 º C, the enzyme dosage of 5 U / mg of sucrose). Scientifically substantiated reasons for reducing the degree of transformation of molasses, sugar syrup strength sorghum. The influence of non-sugars accompanying basic substrates - raw cane sugar, molasses, sweet sorghum syrup, when biocatalytic transformation. Proved inhibitory effect of metal ion (aluminum , iron) , anions of inorganic acids ( nitrate , chloride, phosphate ) , amino acids (serine , aspartic acid -hand ) , organic acids ( citric acid, acetic acid), the process of biotransformation , the formation of sucrose. Reducing the degree of isomerization in the presence of sucrose was observed in the study of non-sugars from 50 to 65 % compared with the control. Isomaltulosesynthase activating effect on , and consequently , the yield of isomaltulose to set calcium and manganese in the form of their sulfates. The introduction of these components into a solution of pure sucrose increases the yield of isomaltulose by 30.5 and 13.2 % respectively. The data obtained will be the basis of studies to optimize the process of biotransformation of various sources of vegetable raw materials rich in sucrose to produce isomaltulose.

biotransformation, sucrose, Erwinia rhapontici, isomaltulose, isomaltulosesyntase, metals, amino acids, organic acids

1. 1 Kawaguti H.Y., Manrich E., Sato H.H. Application of response surface methodology for glucosyltrasferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells [Electronic J. Biotechnology], 2006, vol. 9, no. 5, pp. 482 - 493.

2. 2 Krastanov A., Blazheva D., Yanakieva I., Kratchanova M. Conversion of sucrose into palati-nose in a batch and continuous processes by immobilized Serratia plymutica cells [Enzyme and Mi-crobial Technology], 2006, vol. 39, pp. 1306 - 1312.

3. 3 Korneeva O.S., Bozhko O.Iu., Shuvaeva G.P. Biotechnology isomaltulose - natural sweetener with prebiotic properties. Biotekhnologiia. [Bio-technology], 2008. no. 2, pp. 46-50. (In Russ.).

4. 4 Korneeva O.S., Bozhko O.Iu. Application isomaltulosesynthase Erwinia rhapontici to trans-form sucrose to isomaltulose. Vestnik OGU. [Bulletin of OSU], 2009, no. 4, pp. 130-134. (In Russ.).