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INVESTIGATION OF METHODS OF DNA EXTRACTION FROM PLANT ORIGIN OBJECTS AND FOODS BASED ON THEM

https://doi.org/10.20914/2310-1202-2014-3-142-145

Abstract

For the last decades modern and highly efficient methods of determining the quality and safety of food products, based on the application of the latest scientific achievements were developed in the world. A special place is given to the methods based on achievements of molecular biology and genetics. At the present stage of development in the field of assessing the quality of raw materials and processed food products much attention is given to highly accurate, sensitive and specific research methods, the method of polymerase chain reaction (PCR) occupying a leading place among them. PCR is a sophisticated method that simulates the natural DNA replication and allows to detect a single specific DNA molecule in the presence of millions of other molecules. The key point in the preparation of material for PCR is the extraction of nucleic acids. The low content of DNA in plant material and the high concentration of secondary metabolites complicate the process of extraction. The key solution to this problem is highly effective method of extraction, which allows to obtain the DNA of adequate quality and purity. Comparative analysis of methods for the extraction of nucleic acids from fruit raw materials and products based on them was carried out in the study. General analysis of the experimental data allowed us to determine the most efficient method for DNA extracting. In the comparative analysis it was found out that to extract DNA from plant raw materials and food products prepared on their basis it is the most suitable to use "Sorb-GMO-A" reactants kit (set). The approach described gives us a brilliant opportunity to obtain deoxyribonucleic acid proper quality and purity.

About the Authors

L. S. Dyshlyuk
Kemerovo Institute of Food Science and Technology
Russian Federation
Head of research and educational center


Y. V. Golubtsova
Kemerovo Institute of Food Science and Technology
Russian Federation
senior Lecturer Department of bionanotechnology


M. V. Novoselov
Kemerovo Institute of Food Science and Technology
Russian Federation
graduate student senior Lecturer Department of bionanotechnology


K. A. Shevyakova
Kemerovo Institute of Food Science and Technology
Russian Federation
graduate student Department of bionanotechnology


References

1. 1 Glik B., Pasternak D. Molekuliarnaia biotekhnologiia. Printsipy i primenenie [Molecular biotechnology Principles and application]. Moscow, Mir, 2002. 589 p. (In Russ.).

2. 2 Zorina V.V. Osnovy polimeraznoi tsepnoi reaktsii [The basic of polymerase chain reaction]. Moscow, Mir, 2012. 78 p. (In Russ.).

3. 3 Rasol’ko L. A. Quality as the factor of con-sumer preferences. Molochnyi product. [Dairy product], 2008, no. 2, pp. 26 (In Russ.).

4. 4 Lukashov V.V. Molekuliarnaia evoliutsiia i filogeneticheskii analiz [Molecular evolution and phylogenetic analysis]. Moscow, Binom, 2009. 92 p. (In Russ.).

5. 5 Nikitina E.V., Reshetnik O.A. Bezopasnost’ pishchevykh produktov [Food safety]. Kazan’, 2006. 92 p. (In Russ.).

6. 6 Prosekov A.Iu., Babich O.O., Sukhikh S.A. Sovremennye metody issledovaniia syria i bio-tekhnologicheskoi produktsii [Modern methods of research of raw materials and biotechnology products]. Kemerovo, 2013. 183 p. (In Russ.).

7. 7 Prosekov A.Iu., Babich O.O. Gennaia inzheneriia [Genetic engineering]. Moscow, Dostizheniia nauki i tekhniki APK, 2010. 216 p. (In Russ.).

8. 8 Shaulina L.P., Korsun L.N Kontrol’ kachestva i bezopasnosti pishchevykh produktov i prodovol’stvennogo syr’ia [Quality control and safety of food products and raw materials]. Irkutsk, Izdvo IGU, 2011. 111 p. (In Russ.).


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For citations:


Dyshlyuk L.S., Golubtsova Y.V., Novoselov M.V., Shevyakova K.A. INVESTIGATION OF METHODS OF DNA EXTRACTION FROM PLANT ORIGIN OBJECTS AND FOODS BASED ON THEM. Proceedings of the Voronezh State University of Engineering Technologies. 2014;(3):142-145. (In Russ.) https://doi.org/10.20914/2310-1202-2014-3-142-145

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ISSN 2226-910X (Print)
ISSN 2310-1202 (Online)