The efficiency of synthesis of β-fructofuranosidase by Debaryomyces hansenii yeast when cultured on a nutrient medium from molasses
https://doi.org/10.20914/2310-1202-2016-1-191-197
Abstract
Distribution D. hansenii in different substrates can be explained sufficiently high hydrolytic activity, which is a prerequisite for their use in biotechnology to produce various biological products, in particular lytic enzymes. The paper is picked productivity and efficiency of synthesis of extracellular enzyme β-fructofuranosidase D. hansenii yeast nutrient medium from molasses. The efficiency of D. hansenii yeast culture in a nutrient medium of molasses depends primarily on the strain. It was found that the most productive and efficient synthesizing extracellular enzyme β-fructofuranosidase is yeast strain D. hansenii H4651. Maximum productivity and efficiency of the synthesis of the extracellular enzyme β-fructofuranosidase D. hansenii yeast strain H4651 seen when cultured in a nutrient medium from molasses without additional nutrients at a temperature of 20 oC. It was found that the consumption of reducing substances is not an adequate amount of yeast biomass synthesized D. hansenii. The content of reducing substances after 50 hours of cultivation D. hansenii yeast H4651 Uwe-creases. The resulting pattern PB increase in liquid culture may explain synthesis drozh-Jamie D. hansenii β – fructofuranosidase extracellular enzyme which hydrolyzes sucrose medium. The highest specific growth rate observed in yeast D. hansenii H4651 at temperatures cultivated-tion 15 and 20 ° C. Out D. hansenii yeast biomass H4651 exceeds the yield of biomass when cultured yeast D. hansenii strains H433 and H18–3.
About the Authors
L. Tuan
Kazan national research technological university
Russian Federation
Graduate, Department of food biotechnology. phone (843) 231–89–13
Russian Federation
Graduate, Department of food biotechnology. phone (843) 231–89–13
A. V. Kanarskii
Kazan national research technological university
Russian Federation
professor, Department of food biotechnology. phone (843) 231–89–13
Russian Federation
professor, Department of food biotechnology. phone (843) 231–89–13
Z. A. Kanarskaya
Kazan national research technological university
Russian Federation
associate professor, Department of food biotechnology. phone (843) 231–89–13
Russian Federation
associate professor, Department of food biotechnology. phone (843) 231–89–13
T. V. Sviridova
Voronezh state university of engineering technologies
Russian Federation
Russian Federation
associate professor, Department of biochemistry and biotechnology. phone (473) 255–07–51
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Review
For citations:
Tuan L., Kanarskii A.V., Kanarskaya Z.A., Sviridova T.V. The efficiency of synthesis of β-fructofuranosidase by Debaryomyces hansenii yeast when cultured on a nutrient medium from molasses. Proceedings of the Voronezh State University of Engineering Technologies. 2016;(1):191-197. (In Russ.) https://doi.org/10.20914/2310-1202-2016-1-191-197
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