Influence of alkaline and environmental processing of oil and grain outs on Beta-glucane output

Abstract

Beta-glucan is a branched polysaccharide whose monomers are bound by (1,3, 1,4) and (1,6) beta-glucosidic bonds and has a wide spectrum of biological activity and, above all, immunomodulating and anti-inflammatory. Beta-glucan is found in fungi, yeast, bacteria, algae. However, the isolation of beta-glucan from this raw material, both natural and obtained in industrial conditions by biotechnological methods, is economically costly. A promising source of beta-glucan are grains: oats, barley, wheat, rye, rice, corn and millet. The industrial interest in cereals is associated with a high content of soluble branched non-starchy polysaccharides, from which beta-glucan production is possible. The purpose of this work is to determine the effect of alkaline and two-step alkaline and enzymatic methods for treating oat and oat bran grains on the efficiency of beta-glucan release. As a result of the studies, it has been established that the combination of alkaline and enzymatic methods for the isolation of beta glucan from the grain of oats of holed grinded and oat bran is more effective than the alkaline method. The yield of beta-glucan in the treatment with the alkaline and enzymatic method of oat bran is higher than that from the grain of oats of the hollow grinded. It has been shown that beta-glucan isolated from oat bran by two-step alkaline and enzymatic one-step alkaline methods contains fewer concomitant substances compared to beta-glucan isolated from the grain of oats of the hollow grinded. The higher yield of beta-glucan from oat bran can be explained by the high content in them, and also by the use of a grain-sized grain. The preliminary dissolution of the proteins of the aleuron layer and endosperm in sodium hydroxide, as well as starch and subsequent enzymatic hydrolysis thereof, leads to a more complete recovery of beta-glucan.