Preview

Proceedings of the Voronezh State University of Engineering Technologies

Advanced search

Patterns of inhibition of the culture of Salmonella

https://doi.org/10.20914/2310-1202-2018-4-209-212

Abstract

Studies on the inhibition effectiveness of the initial degree of inoculation of the Salmonella microorganism were carried out. In this paper, the irradiation efficiency of model systems with electron beams with beam energy of 6.5 and 10 MeV was studied. The investigations were carried out at UELV-10-10-C-70 accelerator at Frumkin Institute of Physical Chemistry and Electrochemistry of Russian Academy of Sciences (IPCE RAS) with an average beam power of 6.5 MeV and at radiation-technological center with an electron accelerator UELR-10-10-40 at A.I. Burnazyan Federal Medical Biophysical Centre of Federal Medical Biological Agency with an average electron energy of 10 MeV. The conducted researches on studying and revealing the dependence of inhibition of pathogenic microflora, irradiation with different intensity, on the structure (density) of the studied samples, which model liquid and solid nutrient media. The study used a strain Salmonella enterica subsp. Enterica serovar Typhimurium. The results of the effective inhibition of the initial degree of contamination for the two plants were obtained. Studies have shown that the effectiveness of inhibition of Salmonella culture can vary depending on the characteristics of electron beams. For example, when samples were irradiated with electrons with energy of 6.5 and 10 MeV at doses from 3 to 7 kGy, various results of the inhibition effectiveness of Salmonella culture on media with different work were obtained. When processing samples with studied strains of cultures in the dose range from 4 to 5 kGy, there is an increase in the growth of microorganisms for all processing conditions. In the remaining studied ranges their inhibition are observed. It is important to take into account not only the effectiveness of the oppression of microflora on specific products, but also the efficiency of the installation for a specific sample.

About the Authors

N. V. Ilyuhina
All-Russia Research Institute of Preservation Technology
Cand. Sci. (Chem.), ,, Shkolnaya st., 78, Vidnoe, 144701, Russia


A. Yu. Kolokolova
All-Russia Research Institute of Preservation Technology
Cand. Sci. (Chem.), ,, Shkolnaya st., 78, Vidnoe, 144701, Russia


References

1. Aleksahin R.M., Sanzharova N.I., Koz'min G.V., Geras'kin S.A. et al. Prospects for the use of radiation technologies in the agro-industrial complex of the Russian Federation. Sel'skoe hozyajstvo [Agriculture]. 2014. no. 1. pp.78–85. (in Russian).

2. Farkas J., Mohachi-Farkas C. History and future of food irradiation. Sci. and Tech. 2011. vol. 22. no. 11. pp. 121–126.

3. Molin A.A. Razvitie normativnogo regulirovaniya i populyarizacii primeneniya radiacionnyh tekhnologij v oblasti pishchevoj promyshlennosti [Development of regulatory regulation and popularization of the use of radiation technologies in the food industry. United Innovation Corporation State Enterprise "Rosatom"]. 2012. (in Russian).

4. Frumkin M.L., Koval'skaya L.P., Gel'fand S.Yu. Tekhnologicheskie osnovy radiacionnoj obrabotki pishchevyh produktov [Technological basics of food radiation processing]. Moscow, 1973. 406 p. (in Russian).

5. Petrov A.N., Shishkina N.S., Karastoyanova O.V., Klyueva O.A. et al. The use of ionizing radiation to optimize the technology of refrigeration storage of fruits and vegetables. Holodil'naya tekhnika [Refrigeration]. 2015. no. 11. pp. 51–55. (in Russian).

6. Budnik S.V., Trofimov V.I., SHishkina N.S., Beleckij S.L. Prospects for processing technologies of accelerated electrons of plant materials. Innovacionnye tekhnologii proizvodstva i hraneniya material'nyh cennostej dlya gosudarstvennyh nuzhd [Innovative technologies for the production and storage of wealth for state needs: international collection of scientific articles. FSBI SRIPS Rosrezrerva. under total ed. S.E. Ulanina]. Moscow, Halley-Print, 2014. pp.70–79. (in Russian).

7. Romero S.B., Knotek Z., Cizek A., Masarikova M. et al. The incidence and antibiotic resistance of Salmonella species isolated from cloacae of captive veiled chameleons. Acta Veterinaria Brno. 2015. vol. 84. no. 3. pp. 209–213.

8. Spinali S., van Belkum A., Goering R.V., Girard V. et al. Microbial typing by matrix-assisted laser desorption ionization–time of flight mass spectrometry: do we need guidance for data interpretation? Journal of clinical microbiology. 2015. vol. 53. no. 3. pp. 760–765.

9. Fagerquist C.K., Zaragoza W.J. Proteolytic surface-shaving and serotype-dependent expression of SPI-1 invasion proteins in Salmonella enterica subspecies enterica. Frontiers in Nutrition. 2018. vol. 5. pp. 124.

10. Li B.Z., Chen F., Guo Z.Z., He C.H. et al. Isolation and identification of Salmonella/Escherichia coli analogues in raw pork by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Journal of Food Safety and Quality. 2018. vol. 9. no. 4. pp. 717–722.


Review

For citations:


Ilyuhina N.V., Kolokolova A.Yu. Patterns of inhibition of the culture of Salmonella. Proceedings of the Voronezh State University of Engineering Technologies. 2018;80(4):209-212. (In Russ.) https://doi.org/10.20914/2310-1202-2018-4-209-212

Views: 530


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2226-910X (Print)
ISSN 2310-1202 (Online)