Determination of the antibacterial activity of flavonoids from callus Scutellaria galericulata L. for the development of a functional curd product
https://doi.org/10.20914/2310-1202-2020-1-178-182
Abstract
The antimicrobial effect of extracts from callus of the common skullcap with the prospect of using in the recipe of a functional curd product for therapeutic and preventive action is studied. It is known that plants of the genus skullcap contain phenolic compounds that cause antimicrobial action, the most common among them are flavonoids-baykalin, scutellarein, hydrolyzed to glucuronic acid and aglycones-baykalein and scutellarein, vogonin, etc. the Study of antimicrobial activity was carried out in accordance with the requirements of The state Pharmacopoeia XI edition. Antimicrobial activity was determined in relation to 15 test cultures by diffusion in agar. The method is based on the assessment of inhibition of growth of test cultures of microorganisms by certain concentrations of the test object. Rod-shaped bacteria (Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Serracia marcescens, Escherichia coli, Morganella morganii, Hafnia alvei, Bacillus cereus), coccus microflora (Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium), different strains of fungi of the genus Candida (Candida non-albicans № 136, 138, 142, 144). The result was evaluated according to the following criteria: “-“ – growth of the test culture (there is no antibacterial effect); “+” – no growth (the antibacterial effect is pronounced); “±” – inhibition of growth (bacteriostatic action).
About the Authors
O. V. Belashova
Kemerovo State Medical University
Russian Federation
senior lecturer, pharmacy department, Voroshilov street., 22a Kemerovo, 650056, Russia
Russian Federation
senior lecturer, pharmacy department, Voroshilov street., 22a Kemerovo, 650056, Russia
A. V. Zaushintsena
Kemerovo State University
Dr. Sci. (Biol.), professor, environmental management and ecology department, Red street., 6 Kemerovo, 650000, Russia
Dr. Sci. (Biol.), professor, environmental management and ecology department, Red street., 6 Kemerovo, 650000, Russia
L. A. Levanova
Kemerovo State Medical University
Dr. Sci. (Med.), professor, microbiology, immunology and virology department, Voroshilov street., 22a Kemerovo, 650056, Russia
Dr. Sci. (Med.), professor, microbiology, immunology and virology department, Voroshilov street., 22a Kemerovo, 650056, Russia
Y. V. Zakharova
Kemerovo State Medical University
Cand. Sci. (Med.), associate professor, microbiology, immunology and virology department, Voroshilov street., 22a Kemerovo, 650056, Russia
Cand. Sci. (Med.), associate professor, microbiology, immunology and virology department, Voroshilov street., 22a Kemerovo, 650056, Russia
A. A. Mar
Kemerovo State Medical University
Cand. Sci. (Pharm.), associate professor, pharmacy department, Voroshilov street., 22a Kemerovo, 650056, Russia
Cand. Sci. (Pharm.), associate professor, pharmacy department, Voroshilov street., 22a Kemerovo, 650056, Russia
L. K. Asyakina
Kemerovo State University
Cand. Sci. (Engin.), associate professor, bionanotechnology department, Red street., 6 Kemerovo, 650000, Russia
Cand. Sci. (Engin.), associate professor, bionanotechnology department, Red street., 6 Kemerovo, 650000, Russia
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Review
For citations:
Belashova O.V., Zaushintsena A.V., Levanova L.A., Zakharova Y.V., Mar A.A., Asyakina L.K. Determination of the antibacterial activity of flavonoids from callus Scutellaria galericulata L. for the development of a functional curd product. Proceedings of the Voronezh State University of Engineering Technologies. 2020;82(1):178-182. (In Russ.) https://doi.org/10.20914/2310-1202-2020-1-178-182
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